Bioprocess development for the production of succinic acid from orange peel waste
Date Issued
June 23, 2016
Abstract
A preliminary study has been conducted for the development of a bioprocess targeting the valorization of
orange peel waste (OPW). Essential oils and pectin were recovered from OPW through distillation, acid hydrolysis
and precipitation with ethanol. Optimal conditions for dilute-acid hydrolysis were investigated via estimation of the
sugars released and fermentation. Hydrolysis conditions of 109 o
C for 20 min and 116 o
C for 10 min using 5 % of
dry raw material produced the highest sugar yields at 0.76 and 0.77 (gtotal sugars gdry raw material-1) respectively. In order to
test the efficiency of enzyme hydrolysis as a pretreatment method for OPW valorization, cellulase production from
T. reesei was investigated, which was maximized following 5 days of cultivation. Furthermore, elemental analysis in
hydrolyzates from dilute-acid hydrolysis and a combination of acid and enzyme hydrolysis was performed. The
results indicate that during acid/enzyme hydrolysis, high concentrations of Mg2+ and Ca2+ ions are liberated in
contrast to dilute-acid hydrolysis. A. succinogenes fermentations of glucose and fructose were performed and the
succinic acid yields achieved were 0.66 (gsuccinic acid gglucose-1) and 0.33 (gsuccinic acid gfructose-1) respectively, while
galactose was not fermented. Overall, OPW may serve as a promising raw material for simultaneous production of
essential oils, pectin and succinic acid.
orange peel waste (OPW). Essential oils and pectin were recovered from OPW through distillation, acid hydrolysis
and precipitation with ethanol. Optimal conditions for dilute-acid hydrolysis were investigated via estimation of the
sugars released and fermentation. Hydrolysis conditions of 109 o
C for 20 min and 116 o
C for 10 min using 5 % of
dry raw material produced the highest sugar yields at 0.76 and 0.77 (gtotal sugars gdry raw material-1) respectively. In order to
test the efficiency of enzyme hydrolysis as a pretreatment method for OPW valorization, cellulase production from
T. reesei was investigated, which was maximized following 5 days of cultivation. Furthermore, elemental analysis in
hydrolyzates from dilute-acid hydrolysis and a combination of acid and enzyme hydrolysis was performed. The
results indicate that during acid/enzyme hydrolysis, high concentrations of Mg2+ and Ca2+ ions are liberated in
contrast to dilute-acid hydrolysis. A. succinogenes fermentations of glucose and fructose were performed and the
succinic acid yields achieved were 0.66 (gsuccinic acid gglucose-1) and 0.33 (gsuccinic acid gfructose-1) respectively, while
galactose was not fermented. Overall, OPW may serve as a promising raw material for simultaneous production of
essential oils, pectin and succinic acid.
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