Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14279/9851
DC FieldValueLanguage
dc.contributor.authorBotsaris, George-
dc.contributor.authorLiapi, Maria-
dc.contributor.authorKakogiannis, Charalambos-
dc.contributor.authorDodd, Christine E R-
dc.contributor.authorRees, Catherine E.D.-
dc.date.accessioned2017-02-23T11:01:52Z-
dc.date.available2017-02-23T11:01:52Z-
dc.date.issued2013-06-03-
dc.identifier.citationInternational Journal of Food Microbiology, 2013, vol. 164, no. 1, pp. 76-80en_US
dc.identifier.issn01681605-
dc.identifier.urihttps://hdl.handle.net/20.500.14279/9851-
dc.description.abstractConventional culture and a rapid phage-PCR method were used to detect Mycobacterium avium subsp. paratuberculosis (MAP) in bulk tank milk (BTM) samples. Only two of 225 samples (0.9%) were found to contain MAP by culture whereas 50 (22%) MAP-positive samples were identified using the phage-PCR assay, including both samples that were MAP-culture positive. Results using the phage-based method for independently tested duplicate samples indicated that the assay is very reproducible (r2=0.897), especially when low levels of mycobacteria are present. A relationship was established between plaque number and the presence of MAP in a sample. A cut-off value was determined allowing identification of MAP-positive samples based on plaque number alone (90% sensitivity, 99% specificity; area under the curve=0.976). These results indicate that the assay is a robust method for screening BTM, providing results within 24h.en_US
dc.formatpdfen_US
dc.language.isoenen_US
dc.relation.ispartofInternational journal of food microbiologyen_US
dc.rights© Elsevieren_US
dc.subjectDetectionen_US
dc.subjectMilken_US
dc.subjectParatuberculosisen_US
dc.subjectPhage-PCR assayen_US
dc.titleDetection of Mycobacterium avium subsp. paratuberculosis in bulk tank milk by combined phage-PCR assay: Evidence that plaque number is a good predictor of MAPen_US
dc.typeArticleen_US
dc.collaborationUniversity of Nottinghamen_US
dc.collaborationCyprus Veterinary Servicesen_US
dc.collaborationCyprus University of Technologyen_US
dc.subject.categoryOther Engineering and Technologiesen_US
dc.journalsSubscriptionen_US
dc.countryUnited Kingdomen_US
dc.countryCyprusen_US
dc.subject.fieldEngineering and Technologyen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1016/j.ijfoodmicro.2013.03.023en_US
dc.relation.issue1en_US
dc.relation.volume164en_US
cut.common.academicyear2013-2014en_US
dc.identifier.spage76en_US
dc.identifier.epage80en_US
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.journal.journalissn20576303-
crisitem.journal.publisherElsevier-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.orcid0000-0003-3197-6535-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
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