Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/9851
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Botsaris, George | - |
dc.contributor.author | Liapi, Maria | - |
dc.contributor.author | Kakogiannis, Charalambos | - |
dc.contributor.author | Dodd, Christine E R | - |
dc.contributor.author | Rees, Catherine E.D. | - |
dc.date.accessioned | 2017-02-23T11:01:52Z | - |
dc.date.available | 2017-02-23T11:01:52Z | - |
dc.date.issued | 2013-06-03 | - |
dc.identifier.citation | International Journal of Food Microbiology, 2013, vol. 164, no. 1, pp. 76-80 | en_US |
dc.identifier.issn | 01681605 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.14279/9851 | - |
dc.description.abstract | Conventional culture and a rapid phage-PCR method were used to detect Mycobacterium avium subsp. paratuberculosis (MAP) in bulk tank milk (BTM) samples. Only two of 225 samples (0.9%) were found to contain MAP by culture whereas 50 (22%) MAP-positive samples were identified using the phage-PCR assay, including both samples that were MAP-culture positive. Results using the phage-based method for independently tested duplicate samples indicated that the assay is very reproducible (r2=0.897), especially when low levels of mycobacteria are present. A relationship was established between plaque number and the presence of MAP in a sample. A cut-off value was determined allowing identification of MAP-positive samples based on plaque number alone (90% sensitivity, 99% specificity; area under the curve=0.976). These results indicate that the assay is a robust method for screening BTM, providing results within 24h. | en_US |
dc.format | en_US | |
dc.language.iso | en | en_US |
dc.relation.ispartof | International journal of food microbiology | en_US |
dc.rights | © Elsevier | en_US |
dc.subject | Detection | en_US |
dc.subject | Milk | en_US |
dc.subject | Paratuberculosis | en_US |
dc.subject | Phage-PCR assay | en_US |
dc.title | Detection of Mycobacterium avium subsp. paratuberculosis in bulk tank milk by combined phage-PCR assay: Evidence that plaque number is a good predictor of MAP | en_US |
dc.type | Article | en_US |
dc.collaboration | University of Nottingham | en_US |
dc.collaboration | Cyprus Veterinary Services | en_US |
dc.collaboration | Cyprus University of Technology | en_US |
dc.subject.category | Other Engineering and Technologies | en_US |
dc.journals | Subscription | en_US |
dc.country | United Kingdom | en_US |
dc.country | Cyprus | en_US |
dc.subject.field | Engineering and Technology | en_US |
dc.publication | Peer Reviewed | en_US |
dc.identifier.doi | 10.1016/j.ijfoodmicro.2013.03.023 | en_US |
dc.relation.issue | 1 | en_US |
dc.relation.volume | 164 | en_US |
cut.common.academicyear | 2013-2014 | en_US |
dc.identifier.spage | 76 | en_US |
dc.identifier.epage | 80 | en_US |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
crisitem.journal.journalissn | 20576303 | - |
crisitem.journal.publisher | Elsevier | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.orcid | 0000-0003-3197-6535 | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
Appears in Collections: | Άρθρα/Articles |
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