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https://hdl.handle.net/20.500.14279/8825
Title: | Pilot investigation of the association between serum stress neuropeptide levels and lymphocyte expression of fas and fas ligand in critical illness | Authors: | Papathanassoglou, Elizabeth Mpouzika, Meropi Giannakopoulou, Margarita Bozas, Evangelos Middleton, Nicos Boti, Sofia Karabinis, Andreas |
Major Field of Science: | Medical and Health Sciences | Field Category: | Health Sciences | Keywords: | Neuropeptides;Apoptosis;Stress;Critical illness;PBMC | Issue Date: | 2015 | Source: | Biological research for nursing, 2015, vol. 17, no. 3, pp. 285-294. | Volume: | 17 | Issue: | 3 | Start page: | 285 | End page: | 294 | Journal: | Biological Research for Nursing | Abstract: | In critical illness, apoptotic loss of immunocytes is associated with immunosuppression. Aim:to explore expression of Fas/Fas ligand (FasL) on B and T cells from critically ill patients without sepsis compared to matched controls and associations with disease severity and neuropeptide Y (NPY), cortisol, adrenocorticotropic hormone (ACTH), and prolactin (PRL) levels. Methods: Repeated-measures correlational design with 36 critically ill patients (14-day follow-up) and 36 controls. Disease severity was assessed using the Multiple Organ Dysfunction Score (MODS) and Multi Organ Failure scale.Fas/FasL values were standardized for viable cell counts. An enzyme-linked immunosorbent assay (NPY) and electrochemiluminescence immunoassay (cortisol, ACTH, and PRL) were employed. Results: Fas and FasL expression on T-helper (p < .0001–.03)and T-cytotoxic cells (p < .0001–.002) and Fas expression on B cells (p < .0001–.03) were higher in patients. MODS severity was associated with FasL expression on cytotoxic T cells (r ¼ .752–.902, p ¼ .023–.037). There was an inverse association between Day 1 NPY levels and Fas expression on T-helper cells (r ¼ .447, p ¼ .019). On the day of maximum severity, we report for the first time an inverse association between NPY levels and FasL expression on helper (r ¼ .733, p ¼ .016) and cytotoxic (r ¼.862, p ¼ .003) T cells. Cortisol levels were positively associated with counts of FasL-positive helper (r ¼ .828) and cytotoxic(r ¼ .544, p < .05) T cells. Conclusion: Results suggest a potential role for stress neuropeptides in lymphocyte survival and activation in critical illness. | URI: | https://hdl.handle.net/20.500.14279/8825 | ISSN: | 15524175 | DOI: | 10.1177/1099800414542871 | Rights: | @The Author(s) | Type: | Article | Affiliation : | Cyprus University of Technology University of West Attica National and Kapodistrian University of Athens Onassis Cardiac Surgery Center |
Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
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