Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/3221
Title: | A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes |
Authors: | Tzamaloukas, Ouranios Goulas, Vlasios Angastinioti Moditi, Chrystalleni Andreou, Anisimova |
Major Field of Science: | Agricultural Sciences |
Field Category: | Animal and Dairy Science |
Keywords: | Additives;Antibiotics;Feed premixes;Medicated feed;Sulpha |
Issue Date: | 11-Jun-2014 |
Source: | Journal of Animal and Feed Sciences, 2014, vol. 23, no. 2, pp. 185-189 |
Volume: | 23 |
Issue: | 2 |
Start page: | 185 |
End page: | 189 |
Journal: | Journal of Animal and Feed Sciences |
Abstract: | A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%-2.69%) and intermediate precision (1.36%-4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry. |
URI: | https://hdl.handle.net/20.500.14279/3221 |
ISSN: | 12301388 |
DOI: | 10.22358/jafs/65708/2014 |
Rights: | © Bentus |
Type: | Article |
Affiliation : | Cyprus University of Technology VitaTrace Nutrition Ltd |
Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
Files in This Item:
File | Description | Size | Format | |
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goulasetal232 (1).pdf | 251.59 kB | Adobe PDF | View/Open |
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