Cultivation of Arthrospira platensis in Brewery Wastewater

Abstract

Cultivation of photosynthetic microorganisms in wastewater is a potential cost-effective method of treating wastewater and simultaneously providing the essential nutrients for high-value biomass production. This study investigates the cultivation of the cyanobacterium Arthrospira platensis in non-diluted and non-pretreated brewery wastewater under non-sterile and alkaline growth conditions. The system’s performance in terms of biomass productivity, pollutant consumption, pigment production and biomass composition was evaluated under different media formulations (i.e., addition of sodium chloride and/or bicarbonate) and different irradiation conditions (i.e., continuous illumination and 16:8 light:dark photoperiod). It was observed that the combination of sodium bicarbonate with sodium chloride resulted in maximum pigment production recorded at the end of the experiments, and the use of the photoperiod led to increased pollutant removal (up to 90% of initial concentrations) and biomass concentration (950 mg/L). The composition of the microbial communities established during the experiments was also determined. It was observed that heterotrophic bacteria dominated by the phyla of Pseudomonadota, Bacillota, and Bacteroidota prevailed, while the cyanobacteria population showcased a dynamic behavior throughout the experiments, as it increased towards the end of cultivation (relative abundance of 10% and 30% under continuous illumination and photoperiod application, respectively). Overall, Arthrospira platensis-based cultivation proved to be an effective method of brewery wastewater treatment, although the large numbers of heterotrophic bacteria limit the usage of the produced biomass to applications such as biofuel and biofertilizer production.