Διαφοροποίηση των παθότυπων του clobodera rostochiensis στην περιοχή Αυγόρου
Date Issued
2012
Author(s)
Abstract
The potato cyst nematodes (PCN), Globodera rostochiensis (Woll) (Gr) and Globodera
pallida Stone (Gp) are soil-borne pathogens that cause severe losses to the potato cultivation
in Cyprus. In order to manage PCN with the use of resistant varieties, the identification of
pathotypes of both species is necessary. In this study, the presence of PCN in the wider area
of Augorou village was investigated, as well as the identification of pathotypes that are
encountered in eight potato fields of the area. Soil samples were used for cyst extraction
following the Fenwick method. Molecular characterization of cysts was performed by PCR
reactions. The PCR products revealed the presence of G. rostochiensis alone, in all the soil
samples tested. Pathotype identification was carried out using the Pathotype Scheme for the
European pathotypes of PCN. In addition, Solanum plants were used as differential hosts,
with a different degree of resistance to the pathotypes of G. rostochiensis: Solanum vernei
VTn 62-33-3, Saturna, S. vernei G-LKS 58-1642-4, S.vernei 65-346/19 and S.vernei KTT 60-
21-19. Variety Spunta was used as a control. Pathotype identification results were evaluated
based on the equation Pf / Pi, where Pf = final population and Pi = initial population of PCN.
In the eight agricultural fileds of the wider area of Augorou village, G. rostochiensis Ro1
was identified as the only pathotype. This pathotype is common in the Kokkinochoria area
where PCN are generally found. Management of Ro1 pathotype can be achieved through
Integrated Pest Management (IPM) using resistant varieties with a high commercial value.
However, the combined use of resistant varieties with nematicides is recommended in order
to avoid the development of any other pathotype / species that might be more aggressive than
Ro1.
pallida Stone (Gp) are soil-borne pathogens that cause severe losses to the potato cultivation
in Cyprus. In order to manage PCN with the use of resistant varieties, the identification of
pathotypes of both species is necessary. In this study, the presence of PCN in the wider area
of Augorou village was investigated, as well as the identification of pathotypes that are
encountered in eight potato fields of the area. Soil samples were used for cyst extraction
following the Fenwick method. Molecular characterization of cysts was performed by PCR
reactions. The PCR products revealed the presence of G. rostochiensis alone, in all the soil
samples tested. Pathotype identification was carried out using the Pathotype Scheme for the
European pathotypes of PCN. In addition, Solanum plants were used as differential hosts,
with a different degree of resistance to the pathotypes of G. rostochiensis: Solanum vernei
VTn 62-33-3, Saturna, S. vernei G-LKS 58-1642-4, S.vernei 65-346/19 and S.vernei KTT 60-
21-19. Variety Spunta was used as a control. Pathotype identification results were evaluated
based on the equation Pf / Pi, where Pf = final population and Pi = initial population of PCN.
In the eight agricultural fileds of the wider area of Augorou village, G. rostochiensis Ro1
was identified as the only pathotype. This pathotype is common in the Kokkinochoria area
where PCN are generally found. Management of Ro1 pathotype can be achieved through
Integrated Pest Management (IPM) using resistant varieties with a high commercial value.
However, the combined use of resistant varieties with nematicides is recommended in order
to avoid the development of any other pathotype / species that might be more aggressive than
Ro1.
Subjects
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