Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/2165
Title: | A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus |
Authors: | Papayiannis, Lambros C. Brown, Judith K. Seraphides, Nicos A. Hadjistylli, Margarita Katis, Nikolaos I. Ioannou, Nicolas |
Major Field of Science: | Agricultural Sciences |
Field Category: | Agricultural Biotechnology |
Keywords: | Bemisia tabaci complex;Haplotype;Mitochondrial COI gene;Real-time PCR;RFLP analysis |
Issue Date: | Dec-2009 |
Source: | Bulletin of Entomological Research, 2009, vol. 99, no. 6, pp. 573-582 |
Volume: | 99 |
Issue: | 6 |
Start page: | 573 |
End page: | 582 |
Journal: | Bulletin of Entomological Research |
Abstract: | A real-time PCR assay based on TaqMan® technology was developed and evaluated for the rapid detection of the B and Q biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). A survey was conducted during 2005-2007 in order to identify the distribution and prevalence of B. tabaci biotypes in Cyprus using the real-time PCR assay. More than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using TaqMan® PCR, and the results of the assay were validated by restriction fragment length polymorphism analysis and DNA sequencing of the mitochondrial cytochrome oxidase I (mtCOI) gene. Two biotypes, B and Q, were identified in the collected plant species on the island. The real-time PCR and RFLP assay consistently yielded the same results, although the real-time assay was more sensitive and less time consuming. Phylogenetic analysis of the mtCOI DNA sequences corroborated the identity of the B and Q biotypes 100% of the time and by phylogenetic analysis the haplotypes grouped, as expected, in the major North African-Mediterranean-Middle Eastern clade of the B. tabaci complex. |
URI: | https://hdl.handle.net/20.500.14279/2165 |
ISSN: | 14752670 |
DOI: | 10.1017/S0007485308006603 |
Rights: | © Cambridge University Press |
Type: | Article |
Affiliation : | Agricultural Research Institute of Cyprus University of Arizona Cyprus University of Technology Aristotle University of Thessaloniki |
Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
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