Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/9364
Title: | HPLC-SPE-NMR characterization of major metabolites in Salvia fruticosa mill. Extract with antifungal potential: Relevance of carnosic acid, carnosol, and hispidulin |
Authors: | Exarchou, Vassiliki Kanetis, Loukas Charalambous, Zenovia Apers, Sandra Pieters, Luc A C Gekas, Vassilis Goulas, Vlasios |
Major Field of Science: | Agricultural Sciences |
Field Category: | Agricultural Biotechnology |
Keywords: | Aspergillus tubingensis;Botrytis cinerea;Greek sage;Penicillium digitatum;diterpenes;flavonoids;phytochemicals;q-NMR |
Issue Date: | 12-Jan-2015 |
Source: | Journal of Agricultural and Food Chemistry, 2015, vol. 63, no. 2, pp. 457-463. |
Volume: | 63 |
Issue: | 2 |
Start page: | 457 |
End page: | 469 |
DOI: | 10.1021/jf5050734 |
Journal: | Journal of Agricultural and Food Chemistry |
Abstract: | Plant pathogenic fungi are considered of significant economic importance for adversely affecting both quantitatively and qualitatively fresh and processed produce. Extracts of Salvia fruticosa were initially screened for their antifungal activity, and the ethyl acetate fraction, being the most active, was further analyzed using HPLC-SPE-NMR hyphenation. The methoxylated flavones hispidulin, salvigenin, and cirsimaritin and the diterpenes carnosic acid, carnosol, and 12-methoxycarnosic acid were identified as the major components of the extract. In addition, the concentration levels of all identified components were determined using q-NMR. The antifungal activity of the crude extract and selected phytochemicals was estimated against the fungal species Aspergillus tubingensis, Botrytis cinerea, and Penicillium digitatum. The estimated MIC and MFC values of the ethyl acetate extract of S. fruticosa, as well as three of its major constituents, carnosic acid, carnosol, and hispidulin, support their antifungal activity, especially against B. cinerea and P. digitatum, suggesting their potential use in food and agricultural systems. |
URI: | https://hdl.handle.net/20.500.14279/9364 |
ISSN: | 00218561 |
DOI: | 10.1021/jf5050734 |
Rights: | © American Chemical Society. Attribution-NonCommercial-NoDerivs 3.0 United States |
Type: | Article |
Affiliation : | Cyprus University of Technology |
Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
CORE Recommender
Sorry the service is unavailable at the moment. Please try again later.
This item is licensed under a Creative Commons License