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https://hdl.handle.net/20.500.14279/9068
Title: | Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture | Authors: | Botsaris, George Swift, Benjamin M. C. Slana, Iva Liapi, Maria Christodoulou, Maritsa Hatzitofi, Maria Christodoulou, Vasiliki Rees, Catherine |
metadata.dc.contributor.other: | Μπότσαρης, Γιώργος Χριστοδούλου, Μαρίτσα Χατζηττοφή, Μαρία |
Major Field of Science: | Agricultural Sciences | Field Category: | Agricultural Biotechnology | Keywords: | PCR;Paratuberculosis;Pasteurised milk;Phage;Powdered infant formula;Rapid methods | Issue Date: | 4-Jan-2016 | Source: | International Journal of Food Microbiology,2016, vol. 216, pp. 91-94 | Volume: | 216 | Start page: | 91 | End page: | 94 | Journal: | International journal of food Microbiology | Abstract: | Surveys from different parts of the world have reported that viable Mycobacterium avium subsp. paratuberculosis (MAP) can be cultured from approximately 2% of samples of retail pasteurised milk samples. Pasteurised milk is used for the production of powdered infant formula (PIF) and therefore there is a concern that MAP may also be present in these products. Several studies have previously reported the detection of MAP in PIF using PCR-based assays. However, culture-based surveys of PIF have not detected viable MAP. Here we describe a phage amplification assay coupled with PCR (page-PCR) that can rapidly detect viable MAP in PIF. The results of a small survey showed that the phage-PCR assay detected viable MAP in 13% (4/32) of PIF samples. Culture detected viable MAP in 9% (3/32) PIF samples, all of which were also phage-PCR positive. Direct IS. 900 PCR detected MAP DNA in 22% (7/32) of PIF samples. The presence of viable MAP in PIF indicates that MAP either survived PIF manufacturing or that post-production contamination occurred. Irrespective of the route of MAP contamination, the presence of viable MAP in PIF is a potential public health concern. | URI: | https://hdl.handle.net/20.500.14279/9068 | ISSN: | 18793460 | DOI: | 10.1016/j.ijfoodmicro.2015.09.011 | Rights: | © Elsevier | Type: | Article | Affiliation : | Cyprus University of Technology University of Nottingham Veterinary Research Institute Cyprus Veterinary Services |
Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
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File | Description | Size | Format | |
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Botsaris.pdf | This is a RoMEO green journal | 250.6 kB | Adobe PDF | View/Open |
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