Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/9068
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Botsaris, George | - |
dc.contributor.author | Swift, Benjamin M. C. | - |
dc.contributor.author | Slana, Iva | - |
dc.contributor.author | Liapi, Maria | - |
dc.contributor.author | Christodoulou, Maritsa | - |
dc.contributor.author | Hatzitofi, Maria | - |
dc.contributor.author | Christodoulou, Vasiliki | - |
dc.contributor.author | Rees, Catherine | - |
dc.contributor.other | Μπότσαρης, Γιώργος | - |
dc.contributor.other | Χριστοδούλου, Μαρίτσα | - |
dc.contributor.other | Χατζηττοφή, Μαρία | - |
dc.date.accessioned | 2017-01-17T06:49:58Z | - |
dc.date.available | 2017-01-17T06:49:58Z | - |
dc.date.issued | 2016-01-04 | - |
dc.identifier.citation | International Journal of Food Microbiology,2016, vol. 216, pp. 91-94 | en_US |
dc.identifier.issn | 18793460 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.14279/9068 | - |
dc.description.abstract | Surveys from different parts of the world have reported that viable Mycobacterium avium subsp. paratuberculosis (MAP) can be cultured from approximately 2% of samples of retail pasteurised milk samples. Pasteurised milk is used for the production of powdered infant formula (PIF) and therefore there is a concern that MAP may also be present in these products. Several studies have previously reported the detection of MAP in PIF using PCR-based assays. However, culture-based surveys of PIF have not detected viable MAP. Here we describe a phage amplification assay coupled with PCR (page-PCR) that can rapidly detect viable MAP in PIF. The results of a small survey showed that the phage-PCR assay detected viable MAP in 13% (4/32) of PIF samples. Culture detected viable MAP in 9% (3/32) PIF samples, all of which were also phage-PCR positive. Direct IS. 900 PCR detected MAP DNA in 22% (7/32) of PIF samples. The presence of viable MAP in PIF indicates that MAP either survived PIF manufacturing or that post-production contamination occurred. Irrespective of the route of MAP contamination, the presence of viable MAP in PIF is a potential public health concern. | en_US |
dc.format | en_US | |
dc.language.iso | en | en_US |
dc.relation.ispartof | International journal of food Microbiology | en_US |
dc.rights | © Elsevier | en_US |
dc.subject | PCR | en_US |
dc.subject | Paratuberculosis | en_US |
dc.subject | Pasteurised milk | en_US |
dc.subject | Phage | en_US |
dc.subject | Powdered infant formula | en_US |
dc.subject | Rapid methods | en_US |
dc.title | Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture | en_US |
dc.type | Article | en_US |
dc.collaboration | Cyprus University of Technology | en_US |
dc.collaboration | University of Nottingham | en_US |
dc.collaboration | Veterinary Research Institute | en_US |
dc.collaboration | Cyprus Veterinary Services | en_US |
dc.subject.category | Agricultural Biotechnology | en_US |
dc.journals | Subscription | en_US |
dc.country | Cyprus | en_US |
dc.country | United Kingdom | en_US |
dc.country | Czech Republic | en_US |
dc.subject.field | Agricultural Sciences | en_US |
dc.publication | Peer Reviewed | en_US |
dc.identifier.doi | 10.1016/j.ijfoodmicro.2015.09.011 | en_US |
dc.relation.volume | 216 | en_US |
cut.common.academicyear | 2019-2020 | en_US |
dc.identifier.spage | 91 | en_US |
dc.identifier.epage | 94 | en_US |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
crisitem.journal.journalissn | 20576303 | - |
crisitem.journal.publisher | Elsevier | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.orcid | 0000-0003-3197-6535 | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
Appears in Collections: | Άρθρα/Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Botsaris.pdf | This is a RoMEO green journal | 250.6 kB | Adobe PDF | View/Open |
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