Μοριακή και μορφολογική ταυτοποίηση ειδών του γένους Alternaria από καρπούς ροδιάς
Date Issued
2015
Author(s)
Advisor
Abstract
"Black heart" or "heart rot" disease of pomegranate fruit, related to the
genus Alternaria, has been reported causing significant qualitative and quantitative
crop losses worldwide. The scope of the present study was the species identification
of the Alternaria species involved in the "black heart" of pomegranates in Greece and
Cyprus. Thus, during 2013, local orchards were surveyed and 82 fungal isolates of the
genus Alternaria from Greece and Cyprus were collected from pomegranates
expressing preharvest rot symptoms. Partial sequencing of the endopolygalacturonase
(EndoPG) gene using the primers PG3 and PG2b, revealed that
preharvest Alternaria pomegranate fruit rots were caused by the species Alternaria
alternata, A. tenuissima, and A. arborescens. More specifically, 30 isolates
were A.alternata, 19 A. tenuissima and 14 A. arborescens. The remaining 19 isolates
were found to belong to the complex A. alternata/A. tenuissima and an accurate
separation at the species level was not possible. In order to fulfill full identification
the remaining isolates were characterized based on morphological characteristics and
more specifically on their sporulation patterns. The combinatorial identification
approach followed herein revealed that the predominant Alternaria species related to
the 'black heart" of pomegranates was A. alternata with frequencies of 50 and 55.9%
in Greece and Cyprus, respectively. The species A. tenuissima and A.
arborescens followed at frequencies of 31.2% and 29.4% and 18.8% and 14.7% in
Greece and Cyprus, respectively.
genus Alternaria, has been reported causing significant qualitative and quantitative
crop losses worldwide. The scope of the present study was the species identification
of the Alternaria species involved in the "black heart" of pomegranates in Greece and
Cyprus. Thus, during 2013, local orchards were surveyed and 82 fungal isolates of the
genus Alternaria from Greece and Cyprus were collected from pomegranates
expressing preharvest rot symptoms. Partial sequencing of the endopolygalacturonase
(EndoPG) gene using the primers PG3 and PG2b, revealed that
preharvest Alternaria pomegranate fruit rots were caused by the species Alternaria
alternata, A. tenuissima, and A. arborescens. More specifically, 30 isolates
were A.alternata, 19 A. tenuissima and 14 A. arborescens. The remaining 19 isolates
were found to belong to the complex A. alternata/A. tenuissima and an accurate
separation at the species level was not possible. In order to fulfill full identification
the remaining isolates were characterized based on morphological characteristics and
more specifically on their sporulation patterns. The combinatorial identification
approach followed herein revealed that the predominant Alternaria species related to
the 'black heart" of pomegranates was A. alternata with frequencies of 50 and 55.9%
in Greece and Cyprus, respectively. The species A. tenuissima and A.
arborescens followed at frequencies of 31.2% and 29.4% and 18.8% and 14.7% in
Greece and Cyprus, respectively.
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