Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/32163
DC Field | Value | Language |
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dc.contributor.author | Michael, Kyriakos | - |
dc.contributor.author | Andreou, Constantina | - |
dc.contributor.author | Markou, Anastasia | - |
dc.contributor.author | Christoforou, Michalakis | - |
dc.contributor.author | Nikoloudakis, Nikolaos | - |
dc.date.accessioned | 2024-03-10T14:43:53Z | - |
dc.date.available | 2024-03-10T14:43:53Z | - |
dc.date.issued | 2024-03-05 | - |
dc.identifier.citation | Plants, 2024 vol. 13 n.5, pp.733 | en_US |
dc.identifier.issn | 22237747 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.14279/32163 | - |
dc.description.abstract | Flow cytometry (FCM) is a widely used technique to study genome size (C-value), but recalcitrant metabolites in grapevines often hinder its efficiency in grapevine research. The aim of the present study was (i) to develop a novel buffer tailormade for the nuclei isolation of grapevines and (ii) to characterize a Cypriot germplasm collection based on C-values. A local cultivar "Xinisteri" was used as a pilot test to evaluate a Sorbitol-based buffer, while sprouting, young, and fully matured leaves were examined to evaluate the developmental parameter. The novel Sorbitol buffer was shown to have a coefficient of variation (CV) of 4.06%, indicating improved properties compared to other commonly used FCM buffers [WPB (7.69%), LB01 (6.69%), and LB (7.13%), respectively]. In addition, a significant variation in genome size between genotypes was found in a comprehensive application with 24 grape varieties. Nucleic content (2C) ranged from 0.577/1C pg for the "Assyrtiko" cultivar up to 0.597/1C pg for the "Spourtiko" cultivar, revealing a 17.6/1C Mbp difference. The lowest coefficient of variation (CV) across all entries was found in the variety "Ofthalmo" (2.29%), while the highest was observed in "Pinot Noir" (3.44%). Anova analysis revealed several distinct clusters, showing that in several cases, C-values can be used as a simple method to distinguish grapevine cultivars. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Plants | en_US |
dc.subject | C-value | en_US |
dc.subject | DNA content | en_US |
dc.subject | Sorbitol | en_US |
dc.subject | Tannins | en_US |
dc.subject | Vitis vinifera | en_US |
dc.title | A Novel Sorbitol-Based Flow Cytometry Buffer Is Effective for Genome Size Estimation across a Cypriot Grapevine Collection | en_US |
dc.type | Article | en_US |
dc.collaboration | Cyprus University of Technology | en_US |
dc.journals | Open Access | en_US |
dc.country | Cyprus | en_US |
dc.subject.field | Agricultural Sciences | en_US |
dc.publication | Peer Reviewed | en_US |
dc.identifier.doi | 10.3390/plants13050733 | en_US |
dc.relation.issue | 5 | en_US |
dc.relation.volume | 13 | en_US |
cut.common.academicyear | 2024-2025 | en_US |
dc.identifier.spage | 733 | en_US |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
crisitem.journal.journalissn | 2223-7747 | - |
crisitem.journal.publisher | MDPI | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.orcid | 0000-0002-6217-5987 | - |
crisitem.author.orcid | 0000-0002-3935-8443 | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
Appears in Collections: | Άρθρα/Articles |
Files in This Item:
File | Size | Format | |
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A Novel Sorbitol-Based.pdf | 4.35 MB | Adobe PDF | View/Open |
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