Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14279/30652
Title: Genome Size Variation across a Cypriot Fabeae Tribe Germplasm Collection
Authors: Charalambous, Iliana 
Ioannou, Nektaria 
Kyratzis, Angelos C. 
Kourtellarides, Dimitrios 
Hagidimitriou, Marianna 
Nikoloudakis, Nikolaos 
Major Field of Science: Agricultural Sciences
Field Category: Environmental Biotechnology
Keywords: C-value;DNA content;flow cytometry;genetic resources;legumes
Issue Date: 27-Mar-2023
Source: Plants, 2023, vol. 12, iss. 7
Volume: 12
Issue: 7
Journal: Plants 
Abstract: DNA content is an important trait linked to the evolutionary routes of taxa and often connected to speciation. In the present study, we studied C-values variation across the Cypriot Fabeae gene pool. Several hundred plants (Vicia spp., Lens spp., Pisum spp.) were sampled across Cyprus. Accurate estimates were established by flow cytometry and propidium iodine staining for 155 discrete populations/accessions. A ten-fold variation was detected across lineages with 1C DNA content varying from 1.584 pg for V. cretica (ARI02420) to 13.983 pg for V. faba (ARI00187). In general, flow cytometry was precise for the characterization of species, even though there were instances of genome overlapping across taxa. Most analyses in the current work refer to species that have not been characterized before by flow cytometry (or any other DNA content estimation method). Still, a correlation to C-values previously reported in Kew Plant DNA C-values database was attempted. A high degree of correlation except for V. dalmatica was established. The evaluation of genome size trait in relation with the Fabeae phylogeny, revealed that Pisum and Lens genera were rather homogenous, but an astonishing fluctuation was shown for Vicia spp. Moreover, it was established that genome up- or down-scaling was not directly linked to speciation drivers. The genomic size measurements presented here could deliver extra quality control for the identification and characterization of taxa in germplasm collections, particularly in cases where species share morphological characters.
URI: https://hdl.handle.net/20.500.14279/30652
ISSN: 22237747
DOI: 10.3390/plants12071469
Rights: © by the authors
Attribution-NonCommercial-NoDerivatives 4.0 International
Type: Article
Affiliation : Cyprus University of Technology 
Agricultural Research Institute-Ministry of Agriculture 
Agricultural University of Athens 
Publication Type: Peer Reviewed
Appears in Collections:Άρθρα/Articles

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