Corrigendum to "Recognition motifs for importin 4 [(L)PPRS(G/P)P] and importin 5 [KP(K/Y)LV] binding, identified by bio-informatic simulation and experimental in vitro validation" [Comput Struct Biotechnol J 20 (2022) 5952-5961]
Date Issued
January 2022
DOI
10.1016/j.csbj.2022.12.038
Abstract
Nuclear translocation of large proteins is mediated through karyopherins, carrier proteins recognizing
specific motifs of cargo proteins, known as nuclear localization signals (NLS). However, only few NLS signals have been reported until now. In the present work, NLS signals for Importins 4 and 5 were identified
through an unsupervised in silico approach, followed by experimental in vitro validation. The sequences
LPPRS(G/P)P and KP(K/Y)LV were identified and are proposed as recognition motifs for Importins 4 and 5
binding, respectively. They are involved in the trafficking of important proteins into the nucleus. These
sequences were validated in the breast cancer cell line T47D, which expresses both Importins 4 and 5.
Elucidating the complex relationships of the nuclear transporters and their cargo proteins is very important in better understanding the mechanism of nuclear transport of proteins and laying the foundation
for the development of novel therapeutics, targeting specific importins.
specific motifs of cargo proteins, known as nuclear localization signals (NLS). However, only few NLS signals have been reported until now. In the present work, NLS signals for Importins 4 and 5 were identified
through an unsupervised in silico approach, followed by experimental in vitro validation. The sequences
LPPRS(G/P)P and KP(K/Y)LV were identified and are proposed as recognition motifs for Importins 4 and 5
binding, respectively. They are involved in the trafficking of important proteins into the nucleus. These
sequences were validated in the breast cancer cell line T47D, which expresses both Importins 4 and 5.
Elucidating the complex relationships of the nuclear transporters and their cargo proteins is very important in better understanding the mechanism of nuclear transport of proteins and laying the foundation
for the development of novel therapeutics, targeting specific importins.
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