Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/20.500.14279/1641
Τίτλος: | Multiphoton microscopy of live tissues with ultraviolet autofluorescence | Συγγραφείς: | Li, Chunqiang Runnels, Judith M. Pitsillides, Costas |
Major Field of Science: | Engineering and Technology | Field Category: | Mechanical Engineering;Materials Engineering | Λέξεις-κλειδιά: | In vivo imaging;Multiphoton-excited fluorescence microscopy (MPM);Tryptophan autofluorescence;Video-rate imaging | Ημερομηνία Έκδοσης: | Μαΐ-2010 | Πηγή: | IEEE journal on selected topics in quantum electronics, 2010, vol. 16, no. 3, pp. 516-523 | Volume: | 16 | Issue: | 3 | Start page: | 516 | End page: | 523 | Περιοδικό: | IEEE journal on selected topics in quantum electronics | Περίληψη: | Current research on multiphoton autofluorescence microscopy is primarily focused on imaging the signal from reduced nicotinamide adenine dinucleatide (NADH) in tissue. NADH levels in cells are useful reporters of metabolic information, as well as early indicators in precancer and cancer diagnosis. While NADH is typically imaged in the 400-500 nm spectral window, the amino acid tryptophan is the major source of tissue fluorescence in the Ultraviolet range. Here, we briefly review current progress in multiphoton autofluorescence imaging of live tissues and cells, and report our recent findings of in vivo mouse skin imaging based on multiphoton excited tryptophan autofluorescence. This new method enables noninvasive imaging of skin tissue at video-rate and allows for the visualization and identification of cellular components in the epidermis, dermis, and muscle layers. It is also possible to image through small blood vessels in the mouse skin and observe circulating leukocytes in situ | URI: | https://hdl.handle.net/20.500.14279/1641 | ISSN: | 15584542 | DOI: | 10.1109/JSTQE.2009.2031619 | Rights: | © IEEE | Type: | Article | Affiliation: | Boston University | Affiliation: | Harvard University Universite Laval Massachusetts General Hospital Boston University Wellman Center for Photomedicine |
Publication Type: | Peer Reviewed |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
CORE Recommender
SCOPUSTM
Citations
29
checked on 9 Νοε 2023
WEB OF SCIENCETM
Citations
25
Last Week
0
0
Last month
0
0
checked on 9 Οκτ 2023
Page view(s)
543
Last Week
0
0
Last month
33
33
checked on 13 Μαρ 2025
Google ScholarTM
Check
Altmetric
Όλα τα τεκμήρια του δικτυακού τόπου προστατεύονται από πνευματικά δικαιώματα