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Πεδίο DCΤιμήΓλώσσα
dc.contributor.authorLi, Chunqiang-
dc.contributor.authorRunnels, Judith M.-
dc.contributor.authorPitsillides, Costas-
dc.date.accessioned2013-02-22T13:32:22Zen
dc.date.accessioned2013-05-17T05:22:28Z-
dc.date.accessioned2015-12-02T10:05:09Z-
dc.date.available2013-02-22T13:32:22Zen
dc.date.available2013-05-17T05:22:28Z-
dc.date.available2015-12-02T10:05:09Z-
dc.date.issued2010-05-
dc.identifier.citationIEEE journal on selected topics in quantum electronics, 2010, vol. 16, no. 3, pp. 516-523en_US
dc.identifier.issn15584542-
dc.identifier.urihttps://hdl.handle.net/20.500.14279/1641-
dc.description.abstractCurrent research on multiphoton autofluorescence microscopy is primarily focused on imaging the signal from reduced nicotinamide adenine dinucleatide (NADH) in tissue. NADH levels in cells are useful reporters of metabolic information, as well as early indicators in precancer and cancer diagnosis. While NADH is typically imaged in the 400-500 nm spectral window, the amino acid tryptophan is the major source of tissue fluorescence in the Ultraviolet range. Here, we briefly review current progress in multiphoton autofluorescence imaging of live tissues and cells, and report our recent findings of in vivo mouse skin imaging based on multiphoton excited tryptophan autofluorescence. This new method enables noninvasive imaging of skin tissue at video-rate and allows for the visualization and identification of cellular components in the epidermis, dermis, and muscle layers. It is also possible to image through small blood vessels in the mouse skin and observe circulating leukocytes in situen_US
dc.language.isoenen_US
dc.relation.ispartofIEEE journal on selected topics in quantum electronicsen_US
dc.rights© IEEEen_US
dc.subjectIn vivo imagingen_US
dc.subjectMultiphoton-excited fluorescence microscopy (MPM)en_US
dc.subjectTryptophan autofluorescenceen_US
dc.subjectVideo-rate imagingen_US
dc.titleMultiphoton microscopy of live tissues with ultraviolet autofluorescenceen_US
dc.typeArticleen_US
dc.affiliationBoston Universityen
dc.collaborationHarvard Universityen_US
dc.collaborationUniversite Lavalen_US
dc.collaborationMassachusetts General Hospitalen_US
dc.collaborationBoston Universityen_US
dc.collaborationWellman Center for Photomedicineen_US
dc.subject.categoryMechanical Engineeringen_US
dc.subject.categoryMaterials Engineeringen_US
dc.journalsSubscriptionen_US
dc.countryUnited Statesen_US
dc.countryCanadaen_US
dc.countryCyprusen_US
dc.subject.fieldEngineering and Technologyen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1109/JSTQE.2009.2031619en_US
dc.dept.handle123456789/54en
dc.relation.issue3en_US
dc.relation.volume16en_US
cut.common.academicyear2009-2010en_US
dc.identifier.spage516en_US
dc.identifier.epage523en_US
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.journal.journalissn1558-4542-
crisitem.journal.publisherIEEE-
crisitem.author.deptDepartment of Mechanical Engineering and Materials Science and Engineering-
crisitem.author.facultyFaculty of Engineering and Technology-
crisitem.author.parentorgFaculty of Engineering and Technology-
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