Προβιοτική και Αντιμικροβιακή Δράση των Οξυγαλακτικών Βακτηρίων
Date Issued
2018
Author(s)
Advisor
Abstract
In the present study were used 43 samples of lactic acid bacteria derived from fermented milk, lees and grapes sorting table.
In this study the 43 samples were characterized, and the experiments performed were: (a) the isolation of pure cultures, (b) Gram staining, (c) catalase test, (d) production of CO2 from glucose and (e) growth at different temperatures.
Additionally, the 43 samples were tested for potential probiotic activity, which in the present study was performed as growth at low pH and in the presence of bile salts. Additionally, the 43 samples were tested for potential antimicrobial activity, which in this study was tested by the ability of the trains to produce bacteriocins and inhibit the growth of three pathogenic microorganisms, i.e. Staphylococcus aureus 6571, Listeria monocytogenes 33411 and Listeria monocytogenes 1994.
In addition the molecular identification of 43 lactic acid bacteria samples was performed to determine the species of lactic acid bacteria that corresponds to each sample.
In this study the 43 samples were characterized, and the experiments performed were: (a) the isolation of pure cultures, (b) Gram staining, (c) catalase test, (d) production of CO2 from glucose and (e) growth at different temperatures.
Additionally, the 43 samples were tested for potential probiotic activity, which in the present study was performed as growth at low pH and in the presence of bile salts. Additionally, the 43 samples were tested for potential antimicrobial activity, which in this study was tested by the ability of the trains to produce bacteriocins and inhibit the growth of three pathogenic microorganisms, i.e. Staphylococcus aureus 6571, Listeria monocytogenes 33411 and Listeria monocytogenes 1994.
In addition the molecular identification of 43 lactic acid bacteria samples was performed to determine the species of lactic acid bacteria that corresponds to each sample.
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