Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14279/14663
Title: Genome and species relationships in genus Avena based on RAPD and AFLP molecular markers
Authors: Drossou, A. 
Katsiotis, Andreas 
Leggett, J M 
Loukas, Michael 
Tsakas, S 
Major Field of Science: Agricultural Sciences
Field Category: AGRICULTURAL SCIENCES;Other Agricultural Sciences;SOCIAL SCIENCES
Keywords: Oats;Avena sativa;Crown rust
Issue Date: Jun-2004
Source: Theoretical and Applied Genetics, Volume 109, Issue 1, June 2004, Pages 48-54
Journal: Theoretical and Applied Genetics 
Abstract: Species and genome relationships among 11 diploid (A and C genomes), five tetraploid (AB and AC genomes) and two hexaploid (ACD genome) Avena taxa were investigated using amplified fragment length polymorphisms (AFLPs) and random amplified polymorphic DNA (RAPD) markers. The two primer pairs used for the AFLP reactions produced a total of 354 polymorphic bands, while 187 reproducible bands were generated using ten RAPD primers. Genetic similarities amongst the entries were estimated using the Jaccard and Dice algorithms, and cluster analyses were performed using UPGMA and neighbor joining methods. Principle coordinate analysis was also applied. The highest cophenetic correlation coefficient was obtained for the Jaccard algorithm and UPGMA clustering method ( r=0.99 for AFLP and r=0.94 for RAPD). No major clustering differences were present between phenograms produced with AFLPs and RAPDs. Furthermore, data produced with AFLPs and RAPDs were highly correlated ( r=0.92), indicating the reliability of our results. All A genome diploid taxa are clustered together according to their karyotype. The AB genome tetraploids were found to form a subcluster within the A(s )genome diploids (AFLPs), indicating their near-autoploid origin. The AC genome tetraploids are clustered to the ACD genome hexaploids. Finally, the C genome diploids form an outer branch, indicating the major genomic divergence between the A and C genomes in Avena.
URI: https://hdl.handle.net/20.500.14279/14663
ISSN: 00405752
DOI: 10.1007/s00122-004-1615-y
Type: Article
Affiliation : Agricultural University of Athens 
Plas Gogerddan 
Publication Type: Peer Reviewed
Appears in Collections:Άρθρα/Articles

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