Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/20.500.14279/1455
Τίτλος: | In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response | Συγγραφείς: | Fan, Zhigang Spencer, Joel A. Pitsillides, Costas Lü, Yan Singh, Gurbakhshish Kim, Pilhan Yun, Seok Hyun Andy Toxavidis, Vasilis Strom, Terry Lin, Charles P. Koulmanda, Maria |
Major Field of Science: | Natural Sciences | Field Category: | Electrical Engineering - Electronic Engineering - Information Engineering | Λέξεις-κλειδιά: | Flow cytometry;Photoacoustic effect;Vivo flow | Ημερομηνία Έκδοσης: | Ιου-2010 | Πηγή: | Nature medicine, 2010, vol. 16, no. 6, pp. 718-722 | Volume: | 16 | Issue: | 6 | Start page: | 718 | End page: | 722 | Περιοδικό: | Nature medicine | Περίληψη: | Here we present methods to longitudinally track islet allograft–infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (Teff cells) and discrimination between natural and induced regulatory T cells (nTreg and iTreg cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance | URI: | https://hdl.handle.net/20.500.14279/1455 | ISSN: | 1546170X | DOI: | 10.1038/nm.2155 | Rights: | © Nature America | Type: | Article | Affiliation: | Boston University | Affiliation: | Harvard University Tufts University Boston University |
Publication Type: | Peer Reviewed |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
CORE Recommender
SCOPUSTM
Citations
133
checked on 14 Μαρ 2024
WEB OF SCIENCETM
Citations
122
Last Week
0
0
Last month
1
1
checked on 29 Οκτ 2023
Page view(s)
571
Last Week
0
0
Last month
31
31
checked on 13 Μαρ 2025
Google ScholarTM
Check
Altmetric
Όλα τα τεκμήρια του δικτυακού τόπου προστατεύονται από πνευματικά δικαιώματα