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https://hdl.handle.net/20.500.14279/1127| Title: | Decay of the transient Cu B-CO complex is accompanied by formation of the heme Fe-CO complex of cytochrome cbb 3-CO at ambient temperature: evidence from time-resolved fourier transform infrared spectroscopy |
| Authors: | Stavrakis, Stavros Varotsis, Constantinos Koutsoupakis, Constantinos |
| metadata.dc.contributor.other: | Σταυράκης, Σταύρος Βαρώτσης, Κωνσταντίνος Κουτσουπάκης, Κωνσταντίνος |
| Major Field of Science: | Natural Sciences |
| Field Category: | Chemical Sciences |
| Keywords: | Carbon monoxide;Copper compounds;Fourier transform infrared spectroscopy;Cytochrome;Heme;Iron |
| Issue Date: | 17-Apr-2002 |
| Source: | Journal of the american chemical society, 2002, vol. 124, no.15, pp. 3814-3815 |
| Volume: | 124 |
| Issue: | 15 |
| Start page: | 3814 |
| End page: | 3815 |
| Journal: | Journal of the American Chemical Society |
| Abstract: | Time-resolved step-scan Fourier infrared spectroscopy has been used to study the CO-bound cbb 3-type cytochrome c oxidase from Pseudomonas stutzeri at room temperature. We observe a single band in the FTIR spectrum at 1956 cm -1 (β-form). The time-resolved data indicate that upon photolysis, CO is transferred from heme b 3 (v CO = 1956 cm -1) to CuB (v CO = 2064 cm -1). The decay of the 2065 cm -1 peak (t 1/2 = 120 ± 16 ms) and the development of the 1956 cm -1 peak (t 1/2 = 144 ± 8 ms ) suggest that formation of the Fe-CO complex is concurrent with the decay of the CuB-CO complex. The intensity ratio of the Fe-CO/CuB-CO (2.15) remains constant for all data points, and thus we conclude that no fraction of CO escapes the binuclear center at 293 K |
| URI: | https://hdl.handle.net/20.500.14279/1127 |
| ISSN: | 27863 |
| DOI: | 10.1021/ja0169825 |
| Rights: | © American Chemical Society Attribution-NonCommercial-NoDerivs 3.0 United States |
| Type: | Article |
| Affiliation: | University of Crete |
| Affiliation : | University of Crete |
| Publication Type: | Peer Reviewed |
| Appears in Collections: | Άρθρα/Articles |
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