Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14279/1018
Title: Rapid in vitro microindexing of viroids in citrus
Authors: Kapari-Isaia, T. 
Kyriakou, A. P. 
Papayiannis, Lambros C. 
Gregoriou, S. 
Psaltis, I. 
Tsaltas, Dimitrios 
metadata.dc.contributor.other: Τσάλτας, Δημήτρης
Major Field of Science: Agricultural Sciences
Field Category: Environmental Biotechnology;AGRICULTURAL SCIENCES;Other Agricultural Sciences
Keywords: Citrus exocortis disease;Citrus spp.;Detection and diagnosis;Etrog citron
Issue Date: Apr-2008
Source: Plant Pathology, 2008, Volume 57, Issue 2, Pages 348-353.
Abstract: A new laboratory technique combining shoot-tip grafting in vitro and biological indexing on indicator plants was explored for the detection of citrus exocortis and related viroids. Three in vitro laboratory methods were used and compared with the classical biological method. With the classical in vivo method, diagnosis is based on the expression of symptoms on indicators 11-14 weeks after inoculation. In contrast, with the first in vitro method, microindexing in vitro of citron seedlings by graft inoculation, diagnosis was possible 12 days after inoculation; with the second method, microindexing in vitro of citron cuttings by graft inoculation, 20 days after inoculation; and with the third method, microindexing in vitro of citron cuttings by injection inoculation, 40 days after inoculation. Inoculated Etrog citron plantlets grown in vitro and tested by RT-PCR showed the same viroid content as the source plants. Of the three in vitro viroid indexing methods, microindexing on cuttings by grafting was easier and more reliable than microindexing either on seedlings or on cuttings by injection.
URI: https://hdl.handle.net/20.500.14279/1018
ISSN: 00320862
DOI: 10.1111/j.1365-3059.2007.01774.x
Type: Article
Affiliation : Middlesex University 
Agricultural Research Institute of Cyprus 
Appears in Collections:Άρθρα/Articles

CORE Recommender
Show full item record

SCOPUSTM   
Citations 50

9
checked on Nov 9, 2023

WEB OF SCIENCETM
Citations

8
Last Week
0
Last month
0
checked on Oct 29, 2023

Page view(s) 50

463
Last Week
5
Last month
11
checked on Jul 23, 2024

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons