Απομόνωση και χαρακτηρισμός αλόφιλων ενδοφυτικών και ριζοσφαιρικών βακτηρίων από φυτά της Αλυκής Ακρωτηρίου, ως πιθανοί παράγοντες προώθησης της ανάπτυξης των φυτών
Date Issued
2017
Author(s)
Advisor
Abstract
Soil salinity is the major cause limiting plant productivity and growth worldwide.
Beside plant growth, salinity also has an impact on microbial activity and diversity.
Saline habitants have a unique microbial population, adapted to such environment.
Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that colonize
plant roots and enhance plant growth by a wide variety of mechanisms. The use of
PGPR is steadily increasing in agriculture and offers an attractive way to replace
chemical fertilizers and supplements. In our investigation, we have isolated, screened
and characterized rhizospheric and endophytic PGPR from plants in saline soil of
Akrotiri Salt Lake in Limassol District, Cyprus. Bacterial isolates were screened
preliminarily for their ability to grow in conditions of high salinity and also, tested for
their attachment ability both in the presence and in the absence of salt. Furthermore,
bacterial isolates that showed the highest growth in the presence of 5% NaCl were
identified through 16S rDNA sequencing. Twenty six (26) isolates were tested for
their PGPR traits such as, nitrogen (N) fixation, phosphorus (P) solubilization and
indole-3-acetic acid (IAA) production. Also, the bacterial isolates were tested for
protease, chitinase, cellulose, pectinase activity and for their antifungal activity
against Fusarium oxysporum and Verticillium dahliae. The present study, suggests
that the use of PGPR isolates as inoculants/biofertilizers is potentially beneficial for
plant growth, in the presence of salt due to the production of indole-3-acetic acid
(IAA), nitrogen (N) fixation, phosphorus (P) solubilization and also having antifungal
activity against phytopathogenic fungi. These specific microorganisms should be
further studied to test their capability to promote plant growth (in planta) under
salinity or drought stress conditions.
Beside plant growth, salinity also has an impact on microbial activity and diversity.
Saline habitants have a unique microbial population, adapted to such environment.
Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that colonize
plant roots and enhance plant growth by a wide variety of mechanisms. The use of
PGPR is steadily increasing in agriculture and offers an attractive way to replace
chemical fertilizers and supplements. In our investigation, we have isolated, screened
and characterized rhizospheric and endophytic PGPR from plants in saline soil of
Akrotiri Salt Lake in Limassol District, Cyprus. Bacterial isolates were screened
preliminarily for their ability to grow in conditions of high salinity and also, tested for
their attachment ability both in the presence and in the absence of salt. Furthermore,
bacterial isolates that showed the highest growth in the presence of 5% NaCl were
identified through 16S rDNA sequencing. Twenty six (26) isolates were tested for
their PGPR traits such as, nitrogen (N) fixation, phosphorus (P) solubilization and
indole-3-acetic acid (IAA) production. Also, the bacterial isolates were tested for
protease, chitinase, cellulose, pectinase activity and for their antifungal activity
against Fusarium oxysporum and Verticillium dahliae. The present study, suggests
that the use of PGPR isolates as inoculants/biofertilizers is potentially beneficial for
plant growth, in the presence of salt due to the production of indole-3-acetic acid
(IAA), nitrogen (N) fixation, phosphorus (P) solubilization and also having antifungal
activity against phytopathogenic fungi. These specific microorganisms should be
further studied to test their capability to promote plant growth (in planta) under
salinity or drought stress conditions.
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