Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H +-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum
Journal
Mycological Research
Date Issued
January 2006
Abstract
Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H +-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H +-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen