Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/9912
Title: | Profiling shifts in protein complement in tomato fruit induced by atmospheric ozone-enrichment and/or wound-inoculation with Botrytis cinerea |
Authors: | Tzortzakis, Nikos G. Taybi, Tahar Antony, Edna Singleton, Ian Borland, Anne M. Barnes, Jeremy D. |
metadata.dc.contributor.other: | Τζωρτζάκης, Νίκος Γ. |
Major Field of Science: | Agricultural Sciences |
Field Category: | Agricultural Biotechnology |
Keywords: | Botrytis cinerea;Modified atmosphere storage;;Ozone;Proteomics;Tomato fruit |
Issue Date: | Apr-2013 |
Source: | Postharvest Biology and Technology, 2013, vol. 78, pp. 67-75 |
Volume: | 78 |
Start page: | 67 |
End page: | 75 |
Journal: | Postharvest Biology and Technology |
Abstract: | To unravel the mechanism by which low level atmospheric ozone-enrichment (0.05μmolmol-1) increases the shelf-life of tomato fruit (Lycopersicon esculentum Mill.) by suppressing the growth of pathogens (Botrytis cinerea), protein yield and composition were examined during and following exposure to the gas at 13°C/95% RH. Ozone-enrichment caused marked changes in protein yield and composition in control tomato fruit and suppressed shifts in the proteome induced by wounding/fungal attack. Wound/fungal-inoculation with B. cinerea resulted in a 7% increase in protein yield, and the down-regulation of at least 32 proteins. A number of proteins affected under ozone and wound/fungal-inoculation treatments are involved in the control of cellular oxidative status. Proteins that may be enhanced under oxidative stress were induced during ozone exposure (e.g. thioredoxin peroxidase-TPX), but suppressed following transfer to 'clean air' (e.g. ascorbate peroxidase-APX1). Constitutively-expressed proteins tended to increase reversibly under ozone-treatment, however proteins involved in ripening such as an enzyme related to ethylene biosynthesis (1-aminocyclopropane-1-carboxylate oxidase-ACO) were markedly reduced in ozone-treated tomato fruit but increased in wound-inoculated fruit. Levels of proteins involved in carbohydrate metabolism, pentose phosphate pathway, terpenoid and flavonoid biosynthesis differentiated among the treatments. The presented dataset makes a central contribution to a comprehensive analysis of the manner in which tomato fruit react to ozone-enrichment and/or pathogen infection during storage/transit. |
URI: | https://hdl.handle.net/20.500.14279/9912 |
ISSN: | 09255214 |
DOI: | 10.1016/j.postharvbio.2012.12.005 |
Rights: | © Elsevier |
Type: | Article |
Affiliation : | Cyprus University of Technology Newcastle University |
Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
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