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https://hdl.handle.net/20.500.14279/9054
Τίτλος: | A collaborative evaluation of LC-MS/MS based methods for BMAA analysis: Soluble bound BMAA found to be an important fraction | Συγγραφείς: | Faassen, Elisabeth J. Antoniou, Maria G. Beekman-Lukassen, Wendy Blahova, Lucie Chernova, Ekaterina Christophoridis, Christophoros Combes, Audrey Edwards, Christine N. Fastner, Jutta Harmsen, Joop Hiskia, Anastasia E. Ilag, Leopold L. Kaloudis, Triantafyllos S. Lopicic, Srdjan Lürling, Miquel Mazur-Marzec, Hanna Meriluoto, Jussi A O Porojan, Cristina Viner-Mozzini, Yehudith Zguna, Nadezda |
metadata.dc.contributor.other: | Αντωνίου, Μαρία Γ. | Major Field of Science: | Medical and Health Sciences | Field Category: | Clinical Medicine | Λέξεις-κλειδιά: | 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC);Daphnia magna;Internal standard;Liquid chromatography-tandem mass spectrometry (LC-MS/MS);N-(2-aminoethyl) glycine (AEG);cycad;hydrophilic interaction liquid chromatography (HILIC);phytoplankton;seafood;α,γ-diaminobutyric acid (DAB);β-N-methylamino- | Ημερομηνία Έκδοσης: | 1-Μαρ-2016 | Πηγή: | Marine Drugs, 2016, vol. 14, no. 3 | Volume: | 14 | Issue: | 3 | Περιοδικό: | Marine Drugs | Περίληψη: | Exposure to β-N-methylamino-L-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D3BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D3BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis. | URI: | https://hdl.handle.net/20.500.14279/9054 | ISSN: | 16603397 | DOI: | 10.3390/md14030045 | Rights: | © Multidisciplinary Digital Publishing Institute | Type: | Article | Affiliation: | Wageningen University Cyprus University of Technology Masaryk University Russian Academy of Sciences Institute of Nanoscience and Nanotechnology PSL Research University Robert Gordon University Federal Environment Agency Alterra Stockholm University Athens Water Supply and Sewerage Company (EYDAP SA) University of Belgrade NIOO-KNAW University of Gdansk Åbo Akademi University Cork Institute of Technology Israel Oceanographic and Limnological Research |
Publication Type: | Peer Reviewed |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
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Αντωνίου Μαρία Γ..pdf | 1.91 MB | Adobe PDF | Δείτε/ Ανοίξτε |
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