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https://hdl.handle.net/20.500.14279/32574
Τίτλος: | Probing the Fucoxanthin-Chlorophyll a/c-Binding Proteins (FCPs) of the Marine Diatom Fragilariopsis sp. by Resonance Raman Spectroscopy | Συγγραφείς: | Andreou, Charalampos Tselios, Charalampos Ioannou, Aristos Varotsis, Constantinos |
Major Field of Science: | Engineering and Technology | Field Category: | Chemical Engineering | Ημερομηνία Έκδοσης: | 26-Οκτ-2023 | Πηγή: | The journal of physical chemistry. B, 2023, vol. 127, iss. 42, pp. 9014–9020 | Volume: | 127 | Issue: | 42 | Start page: | 9014 | End page: | 9020 | Περιοδικό: | The Journal of Physical Chemistry B | Περίληψη: | We report resonance Raman spectra of the light-harvesting fucoxanthin-chlorophyll a/c-binding proteins (FCPs) of marine diatom Fragilariopsis sp. The Raman shifts in the 15N-isotope-enriched diatom provide the first spectroscopic evidence for the characterization of the Ca-N marker bands and, thus, of the penta- and hexacoordinated states of chlorophylls a/c in the FCPs. Under 405 and 442 nm Raman excitations, all of the marker bands of Chl a/c are observed and the isotope-based assignments provide new information concerning the structure of Chls a/c in the FCPs and their interactions with the protein environment. Therefore, the Raman spectrum at 405 nm originates from the π-π* transitions of Chl a/c and not from a different, non π-π* electronic transition, as previously reported (BBA Bioenergetics, 2010, 1797, 1647-1656). Based on the 15N isotope shifts of the Ca-N and in conjunction with other marker bands, two distinct conformations of five- and six-coordinated Chl a and Chl c are observed. In addition, two keto carbonyls were observed at 1679 (strong H-bonded) and 1691 cm-1 (weak H-bonded) in both the 405 and 442 nm Raman spectra, respectively. Collectively, the results provide solid evidence of the nature of the vibrational modes of the active Chl a/c photosynthetic pigments in the FCPs. | URI: | https://hdl.handle.net/20.500.14279/32574 | ISSN: | 15206106 | DOI: | 10.1021/acs.jpcb.3c04346 | Rights: | CC-BY 4.0. Attribution-NonCommercial-NoDerivatives 4.0 International |
Type: | Article | Affiliation: | Cyprus University of Technology | Publication Type: | Peer Reviewed |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
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andreou-et-al-2023-probing-the-fucoxanthin-chlorophyll-a-c-binding-proteins-(fcps)-of-the-marine-diatom-fragilariopsis.pdf | Open Access | 4.98 MB | Adobe PDF | Δείτε/ Ανοίξτε |
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