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https://hdl.handle.net/20.500.14279/3221
Τίτλος: | A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes | Συγγραφείς: | Tzamaloukas, Ouranios Goulas, Vlasios Angastinioti Moditi, Chrystalleni Andreou, Anisimova |
Major Field of Science: | Agricultural Sciences | Field Category: | Animal and Dairy Science | Λέξεις-κλειδιά: | Additives;Antibiotics;Feed premixes;Medicated feed;Sulpha | Ημερομηνία Έκδοσης: | 11-Ιου-2014 | Πηγή: | Journal of Animal and Feed Sciences, 2014, vol. 23, no. 2, pp. 185-189 | Volume: | 23 | Issue: | 2 | Start page: | 185 | End page: | 189 | Περιοδικό: | Journal of Animal and Feed Sciences | Περίληψη: | A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%-2.69%) and intermediate precision (1.36%-4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry. | URI: | https://hdl.handle.net/20.500.14279/3221 | ISSN: | 12301388 | DOI: | 10.22358/jafs/65708/2014 | Rights: | © Bentus | Type: | Article | Affiliation: | Cyprus University of Technology VitaTrace Nutrition Ltd |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
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