Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/29611
Title: | Evidence for reversible light-dependent transitions in the photosynthetic pigments of diatoms | Authors: | Tselios, Charalampos Varotsis, Constantinos |
Major Field of Science: | Engineering and Technology | Field Category: | Chemical Engineering | Keywords: | Blue shift;Chlorophyll;High intensity light;Phytoplankton;Red Shift | Issue Date: | 3-Nov-2022 | Source: | RSC Advances, vol. 12, iss. 49, pp. 31555 - 31563 | Volume: | 12 | Issue: | 49 | Start page: | 31555 | End page: | 31563 | Abstract: | Marine diatoms contribute to oxygenic photosynthesis and carbon fixation and handle large changes under variable light intensity on a regular basis. The unique light-harvesting apparatus of diatoms are the fucoxanthin-chlorophyll a/c-binding proteins (FCPs). Here, we show the enhancement of chlorophyll a/c (Chl a/c), fucoxanthin (Fx), and diadinoxanthin (Dd) marker bands in the Raman spectra of the centric diatom T. pseudonana, which allows distinction of the pigment content in the cells grown under low- (LL) and high-light (HL) intensity at room temperature. Reversible LL-HL dependent conformations of Chl c, characteristic of two conformations of the porphyrin macrocycle, and the presence of five- and six-coordinated Chl a/c with weak axial ligands are observed in the Raman data. Under HL the energy transfer from Chl c to Chl a is reduced and that from the red-shifted Fxs is minimal. Therefore, Chl c and the blue-shifted Fxs are the only contributors to the energy transfer pathways under HL and the blue- to red-shifted Fxs energy transfer pathway characteristic of the LL is inactive. The results indicate that T. pseudonana can redirect its function from light harvesting to energy-quenching state, and reversibly to light-harvesting upon subsequent illumination to LL by reproducing the red-shifted Fxs and decrease the number of Dds. The LL to HL reversible transitions are accompanied by structural modifications of Chl a/c and the lack of the red-shifted Fxs. | URI: | https://hdl.handle.net/20.500.14279/29611 | ISSN: | 20462069 | DOI: | 10.1039/d2ra05284a | Rights: | © The Author(s) Attribution-NonCommercial-NoDerivatives 4.0 International |
Type: | Article | Affiliation : | Cyprus University of Technology | Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
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varotsis.pdf | Full text | 1.04 MB | Adobe PDF | View/Open |
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