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https://hdl.handle.net/20.500.14279/10541
Τίτλος: | Metabolic and transcriptional elucidation of the carotenoid biosynthesis pathway in peel and flesh tissue of loquat fruit during on-tree development | Συγγραφείς: | Hadjipieri, Margarita Georgiadou, Egli C. Marin, Alicia Diaz-Mula, Huertas M. Goulas, Vlasios Fotopoulos, Vasileios Tomás-Barberán, Francisco A. Manganaris, George A. |
Major Field of Science: | Agricultural Sciences | Field Category: | Agriculture Forestry and Fisheries | Λέξεις-κλειδιά: | Eriobotrya japonica;Developmental stages;Maturation;Ripening;Beta-carotene;Beta-cryptoxanthin;Lutein;LC-MS;Biosynthetic pathway | Ημερομηνία Έκδοσης: | 2017 | Πηγή: | BMC Plant Biology, 2017, vol. 17, no. 1 | Volume: | 17 | Issue: | 1 | Περιοδικό: | BMC Plant Biology | Περίληψη: | Background: Carotenoids are the main colouring substances found in orange-fleshed loquat fruits. The aim of this study was to unravel the carotenoid biosynthetic pathway of loquat fruit (cv. 'Obusa') in peel and flesh tissue during distinct on-tree developmental stages through a targeted analytical and molecular approach. Results: Substantial changes regarding colour parameters, both between peel and flesh and among the different developmental stages, were monitored, concomitant with a significant increment in carotenoid content. Key genes and individual compounds that are implicated in the carotenoid biosynthetic pathway were further dissected with the employment of molecular (RT-qPCR) and advanced analytical techniques (LC-MS). Results revealed significant differences in carotenoid composition between peel and flesh. Thirty-two carotenoids were found in the peel, while only eighteen carotenoids were identified in the flesh. Trans-lutein and trans-beta-carotene were the major carotenoids in the peel; the content of the former decreased with the progress of ripening, while the latter registered a 7.2-fold increase. However, carotenoid profiling of loquat flesh indicated trans-acryptoxanthin, followed by trans-beta-carotene and 5,8-epoxy-beta-carotene to be the most predominant carotenoids. High amounts of trans-beta-carotene in both tissues were supported by significant induction in a chromoplast-specific lycopene beta-cyclase (CYCB) transcript levels. PSY1, ZDS, CYCB and BCH were up-regulated and CRTISO, LCYE, ECH and VDE were down-regulated in most of the developmental stages compared with the immature stage in both peel and flesh tissue. Overall, differential regulation of expression levels with the progress of on-tree fruit development was more evident in the middle and downstream genes of carotenoid biosynthetic pathway. Conclusions: Carotenoid composition is greatly affected during on-tree loquat development with striking differences between peel and flesh tissue. A link between gene up-or down-regulation during the developmental stages of the loquat fruit, and how their expression affects carotenoid content per tissue (peel or flesh) was established. | URI: | https://hdl.handle.net/20.500.14279/10541 | ISSN: | 14712229 | DOI: | 10.1186/s12870-017-1041-3 | Rights: | © The Author(s) This article is distributed under the terms of the Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, andreproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver applies to the data made available in this article, unless otherwise stated. | Type: | Article | Affiliation: | Cyprus University of Technology CSIC - Centro de Edafologia y Biologia Aplicada del Segura |
Publication Type: | Peer Reviewed |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
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