Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο: https://hdl.handle.net/20.500.14279/10541
Τίτλος: Metabolic and transcriptional elucidation of the carotenoid biosynthesis pathway in peel and flesh tissue of loquat fruit during on-tree development
Συγγραφείς: Hadjipieri, Margarita 
Georgiadou, Egli C. 
Marin, Alicia 
Diaz-Mula, Huertas M. 
Goulas, Vlasios 
Fotopoulos, Vasileios 
Tomás-Barberán, Francisco A. 
Manganaris, George A. 
Major Field of Science: Agricultural Sciences
Field Category: Agriculture Forestry and Fisheries
Λέξεις-κλειδιά: Eriobotrya japonica;Developmental stages;Maturation;Ripening;Beta-carotene;Beta-cryptoxanthin;Lutein;LC-MS;Biosynthetic pathway
Ημερομηνία Έκδοσης: 2017
Πηγή: BMC Plant Biology, 2017, vol. 17, no. 1
Volume: 17
Issue: 1
Περιοδικό: BMC Plant Biology 
Περίληψη: Background: Carotenoids are the main colouring substances found in orange-fleshed loquat fruits. The aim of this study was to unravel the carotenoid biosynthetic pathway of loquat fruit (cv. 'Obusa') in peel and flesh tissue during distinct on-tree developmental stages through a targeted analytical and molecular approach. Results: Substantial changes regarding colour parameters, both between peel and flesh and among the different developmental stages, were monitored, concomitant with a significant increment in carotenoid content. Key genes and individual compounds that are implicated in the carotenoid biosynthetic pathway were further dissected with the employment of molecular (RT-qPCR) and advanced analytical techniques (LC-MS). Results revealed significant differences in carotenoid composition between peel and flesh. Thirty-two carotenoids were found in the peel, while only eighteen carotenoids were identified in the flesh. Trans-lutein and trans-beta-carotene were the major carotenoids in the peel; the content of the former decreased with the progress of ripening, while the latter registered a 7.2-fold increase. However, carotenoid profiling of loquat flesh indicated trans-acryptoxanthin, followed by trans-beta-carotene and 5,8-epoxy-beta-carotene to be the most predominant carotenoids. High amounts of trans-beta-carotene in both tissues were supported by significant induction in a chromoplast-specific lycopene beta-cyclase (CYCB) transcript levels. PSY1, ZDS, CYCB and BCH were up-regulated and CRTISO, LCYE, ECH and VDE were down-regulated in most of the developmental stages compared with the immature stage in both peel and flesh tissue. Overall, differential regulation of expression levels with the progress of on-tree fruit development was more evident in the middle and downstream genes of carotenoid biosynthetic pathway. Conclusions: Carotenoid composition is greatly affected during on-tree loquat development with striking differences between peel and flesh tissue. A link between gene up-or down-regulation during the developmental stages of the loquat fruit, and how their expression affects carotenoid content per tissue (peel or flesh) was established.
URI: https://hdl.handle.net/20.500.14279/10541
ISSN: 14712229
DOI: 10.1186/s12870-017-1041-3
Rights: © The Author(s) This article is distributed under the terms of the Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, andreproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver applies to the data made available in this article, unless otherwise stated.
Type: Article
Affiliation: Cyprus University of Technology 
CSIC - Centro de Edafologia y Biologia Aplicada del Segura 
Publication Type: Peer Reviewed
Εμφανίζεται στις συλλογές:Άρθρα/Articles

Αρχεία σε αυτό το τεκμήριο:
Αρχείο Περιγραφή ΜέγεθοςΜορφότυπος
document.pdf1.1 MBAdobe PDFΔείτε/ Ανοίξτε
CORE Recommender
Δείξε την πλήρη περιγραφή του τεκμηρίου

SCOPUSTM   
Citations

40
checked on 9 Νοε 2023

WEB OF SCIENCETM
Citations

37
Last Week
0
Last month
2
checked on 29 Οκτ 2023

Page view(s) 50

430
Last Week
0
Last month
4
checked on 22 Δεκ 2024

Download(s)

185
checked on 22 Δεκ 2024

Google ScholarTM

Check

Altmetric


Όλα τα τεκμήρια του δικτυακού τόπου προστατεύονται από πνευματικά δικαιώματα