Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14279/9778
Title: Saccharomyces cerevisiae and Oenococcus oeni immobilized in different layers of a cellulose/starch gel composite for simultaneous alcoholic and malolactic wine fermentations
Authors: Servetas, Ioannis 
Berbegal, Carmen 
Camacho, Nathalia 
Bekatorou, Argyro 
Ferrer, Sergi 
Nigam, Poonam Singh 
Drouza, Chryssoula 
Koutinas, Αthanasios Α. 
Major Field of Science: Agricultural Sciences
Field Category: Other Agricultural Sciences
Keywords: Composite biocatalyst;Immobilization;Malolactic fermentation;Oenococcus oeni;Saccharomyces cerevisiae;Wine
Issue Date: Sep-2013
Source: Process Biochemistry, 2013, vol. 48, no.9, pp. 1279-1284
Volume: 48
Issue: 9
Start page: 1279
End page: 1284
Journal: Process Biochemistry 
Abstract: The production of a two-layer composite biocatalyst for immobilization of two different microorganisms for simultaneous alcoholic and malolactic fermentation (MLF) of wine in the same bioreactor is reported. The biocatalyst consisted of a tubular delignified cellulosic material (DCM) with entrapped Oenococcus oeni cells, covered with starch gel containing the alcohol resistant and cryotolerant strain Saccharomyces cerevisiae AXAZ-1. The biocatalyst was found effective for simultaneous low temperature alcoholic fermentation resulting to conversion of malic acid to lactic acid in 5 days at 10 °C. Improvement of wine quality compared with wine fermented with S. cerevisiae AXAZ-1 immobilized on DCM was attributed to MLF as well as to increased ester formation and lower higher alcohols produced at low fermentation temperatures (10 °C) as shown by GC and headspace SPME GC/MS analysis. Scanning electron microscopy showed that the preparation of a three-layer composite biocatalyst is also possible. The significance of such composite biocatalysts is the feasibility of two or three bioprocesses in the same bioreactor, thus reducing production cost in the food industry.
URI: https://hdl.handle.net/20.500.14279/9778
ISSN: 13595113
DOI: 10.1016/j.procbio.2013.06.020
Rights: © Elsevier
Type: Article
Affiliation : University of Patras 
University of Valencia 
University of Ulster at Coleraine 
Cyprus University of Technology 
Publication Type: Peer Reviewed
Appears in Collections:Άρθρα/Articles

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