Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/20.500.14279/9351
Πεδίο DC | Τιμή | Γλώσσα |
---|---|---|
dc.contributor.author | Georgiadou, Egli C. | - |
dc.contributor.author | Ntourou, Thessaloniki | - |
dc.contributor.author | Goulas, Vlasios | - |
dc.contributor.author | Manganaris, George A. | - |
dc.contributor.author | Kalaitzis, Panagiotis | - |
dc.contributor.author | Fotopoulos, Vasileios | - |
dc.date.accessioned | 2017-02-01T13:42:55Z | - |
dc.date.available | 2017-02-01T13:42:55Z | - |
dc.date.issued | 2015-10-21 | - |
dc.identifier.citation | Frontiers in Plant Science, 2015, vol. 6, no. 871 | en_US |
dc.identifier.issn | 1664462X | - |
dc.identifier.uri | https://hdl.handle.net/20.500.14279/9351 | - |
dc.description.abstract | The aim of this work was to generate a high resolution temporal mapping of the biosynthetic pathway of vitamin E in olive fruit (Olea europaea cv. “Koroneiki”) during 17 successive on-tree developmental stages. Fruit material was collected from the middle of June until the end of January, corresponding to 6–38 weeks after flowering (WAF). Results revealed a variable gene regulation pattern among 6–38 WAF studied and more pronounced levels of differential regulation of gene expression for the first and intermediate genes in the biosynthetic pathway (VTE5, geranylgeranyl reductase, HPPD, VTE2, HGGT and VTE3) compared with the downstream components of the pathway (VTE1 and VTE4). Notably, expression of HGGT and VTE2 genes were significantly suppressed throughout the developmental stages examined. Metabolite analysis indicated that the first and intermediate stages of development (6–22 WAF) have higher concentrations of tocochromanols compared with the last on-tree stages (starting from 24 WAF onwards). The concentration of α-tocopherol (16.15 ± 0.60−32.45 ± 0.54 mg/100 g F.W.) were substantially greater (up to 100-fold) than those of β-, γ-, and δ-tocopherols (0.13 ± 0.01−0.25 ± 0.03 mg/100 g F.W., 0.13 ± 0.01−0.33 ± 0.04 mg/100 g F.W., 0.14 ± 0.01−0.28 ± 0.01 mg/100 g F.W., respectively). In regard with tocotrienol content, only γ-tocotrienol was detected. Overall, olive fruits (cv. “Koroneiki”) exhibited higher concentrations of vitamin E until 22 WAF as compared with later WAF, concomitant with the expression profile of phytol kinase (VTE5), which could be used as a marker gene due to its importance in the biosynthesis of vitamin E. To the best of our knowledge, this is the first study that explores the complete biosynthetic pathway of vitamin E in a fruit tree crop of great horticultural importance such as olive, linking molecular gene expression analysis with tocochromanol content. | en_US |
dc.format | en_US | |
dc.language.iso | en | en_US |
dc.relation.ispartof | Frontiers in Plant Science | en_US |
dc.rights | © 2015 Georgiadou, Ntourou, Goulas, Manganaris, Kalaitzis and Fotopoulos. | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | Olea europaea | en_US |
dc.subject | Developmental stages | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Phytol kinase | en_US |
dc.subject | Tocochromanols | en_US |
dc.subject | Tocopherols | en_US |
dc.subject | Tocotrienols | en_US |
dc.title | Temporal analysis reveals a key role for VTE5 in vitamin E biosynthesis in olive fruit during on-tree development | en_US |
dc.type | Article | en_US |
dc.doi | 10.3389/fpls.2015.00871 | en_US |
dc.collaboration | Cyprus University of Technology | en_US |
dc.collaboration | Mediterranean Agronomic Institute Chania | en_US |
dc.subject.category | Agriculture Forestry and Fisheries | en_US |
dc.journals | Open Access | en_US |
dc.country | Cyprus | en_US |
dc.country | Greece | en_US |
dc.subject.field | Agricultural Sciences | en_US |
dc.publication | Peer Reviewed | en_US |
dc.identifier.doi | 10.3389/fpls.2015.00871 | en_US |
dc.identifier.pmid | 26557125 | - |
dc.relation.issue | 871 | en_US |
dc.relation.volume | 6 | en_US |
cut.common.academicyear | 2015-2016 | en_US |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
crisitem.journal.journalissn | 1664-462X | - |
crisitem.journal.publisher | Frontiers | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.orcid | 0000-0002-5073-979X | - |
crisitem.author.orcid | 0000-0001-7527-1559 | - |
crisitem.author.orcid | 0000-0002-5849-6104 | - |
crisitem.author.orcid | 0000-0003-1205-2070 | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
Αρχεία σε αυτό το τεκμήριο:
Αρχείο | Περιγραφή | Μέγεθος | Μορφότυπος | |
---|---|---|---|---|
Georgiadou.pdf | Article | 3.83 MB | Adobe PDF | Δείτε/ Ανοίξτε |
CORE Recommender
SCOPUSTM
Citations
18
checked on 14 Μαρ 2024
WEB OF SCIENCETM
Citations
20
18
Last Week
0
0
Last month
0
0
checked on 29 Οκτ 2023
Page view(s)
494
Last Week
0
0
Last month
34
34
checked on 13 Μαρ 2025
Download(s) 20
165
checked on 13 Μαρ 2025
Google ScholarTM
Check
Altmetric
Αυτό το τεκμήριο προστατεύεται από άδεια Άδεια Creative Commons