The importance of hydrogen sulfide as a systemic priming agent in strawberry plants grown under key abiotic stress factors
Date Issued
2011
Abstract
Hydrogen sulfide (H2S) is an endogenous gasotransmitter which has been recently
found to playa major signaling role in response to abiotic stress factors. In the present
study we tested whether hydroponic pre-treatment of strawberry (Fragaria x ananassa
cv. Camarosa) roots to a H2S donor, NaHS (10 mM for 48 h) could induce long lasting
priming effects and tolerance to subsequent exposure to 100 mM NaCI or 10% (w/v)
PEG-6000 for 7 d, as well as to heat stress (42° C) applied for 8 h. This task was
undertaken by employing a variety of physiological, biochemical and molecular
approaches. Hydrogen sulfide pre-treatment of roots resulted in significantly increased
leaf chlorophyll fluorescence, stomatal conductance and relative leaf water content as
well as reduced ion leakage and lipid peroxidation levels in comparison with plants
directly subjected to salt, PEG and heat stress, suggesting a systemic mitigating effect
of H2S pre-treatment to cellular damage derived from abiotic stress factors. In addition,
root pre-treatment resulted in the minimization of oxidative and nitrosative stress in
strawberry plants, manifested via the reduced de novo synthesis of NO and H202 in
leaves and the maintenance of high ascorbate and glutathione redox states following
subsequent salt and hyperosmotic stresses. Quantitative real-time RT-PCR data
examining antioxidant, transcription factor, heat shock protein and ion transporter gene
expression levels suggest that H2S plays a key role in the regulation of multiple
transcriptional pathways. Our results indicate that H2S pre-treated plants managed to
overcome the deleterious effects of salt, heat and hyperosmotic stress, by controlling
oxidative and nitrosative cellular damage mainly through increased performance of
antioxidant mechanism, thus proposing a novel role for H2S in plant priming.
found to playa major signaling role in response to abiotic stress factors. In the present
study we tested whether hydroponic pre-treatment of strawberry (Fragaria x ananassa
cv. Camarosa) roots to a H2S donor, NaHS (10 mM for 48 h) could induce long lasting
priming effects and tolerance to subsequent exposure to 100 mM NaCI or 10% (w/v)
PEG-6000 for 7 d, as well as to heat stress (42° C) applied for 8 h. This task was
undertaken by employing a variety of physiological, biochemical and molecular
approaches. Hydrogen sulfide pre-treatment of roots resulted in significantly increased
leaf chlorophyll fluorescence, stomatal conductance and relative leaf water content as
well as reduced ion leakage and lipid peroxidation levels in comparison with plants
directly subjected to salt, PEG and heat stress, suggesting a systemic mitigating effect
of H2S pre-treatment to cellular damage derived from abiotic stress factors. In addition,
root pre-treatment resulted in the minimization of oxidative and nitrosative stress in
strawberry plants, manifested via the reduced de novo synthesis of NO and H202 in
leaves and the maintenance of high ascorbate and glutathione redox states following
subsequent salt and hyperosmotic stresses. Quantitative real-time RT-PCR data
examining antioxidant, transcription factor, heat shock protein and ion transporter gene
expression levels suggest that H2S plays a key role in the regulation of multiple
transcriptional pathways. Our results indicate that H2S pre-treated plants managed to
overcome the deleterious effects of salt, heat and hyperosmotic stress, by controlling
oxidative and nitrosative cellular damage mainly through increased performance of
antioxidant mechanism, thus proposing a novel role for H2S in plant priming.
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