Oxidative and nitrosative-based signaling wave and posttranslational modification orchestrates the acclimation of citrus plants to salinity stress
Date Issued
November 17, 2011
Abstract
Reactive oxygen species and reactive nitrogen species involved in a plethora of
physiologic and pathologic conditions in plants, however knowledge on the oxidative
and nitrosative signaling outcomes is still unclear. To better understand how oxidative
and nitrosative signals are integrated to eventually regulate cellular adjustments to
external conditions, local and systemic responses were investigated in roots and
leaves of citrus plants after root treatment with H202 or sodium nitroprusside (SNP;
nitric oxide donor), pre-treatment with H202 or SNP followed by NaCI stress, and
direct NaCI stress for 8 days. Phenotypic and physiological data showed that preexposure
to H202 or SNP induced acclimation to subsequent salinity stress. Both
H202 and NO were locally and systemically accumulated in the citrus's tissues upon
chemical treatments and/or NaCl. Combined histochemical and fluorescent
approaches document the existence of a vascular tissue-driven long distance ROS and
NO signaling mechanism. Transcriptional analysis of genes diagnostic for H202 and
NO signaling just after chemical treatments or following 8 days of salinity revealed
various tissue- and time- specific feedbacklfeedforward mechanisms controlling
internal H202 and NO homeostasis. Also evidence presented showing that protein
carbonylation, nitration and S-nitrosylation encode an 'oxidative and nitrosative
memory' following by H202 or SNP pre-treatments that could be involved in
acclimation to salt stress. In addition, for the first time, a whole-plant analysis of the
oxyproteome, nitroproteome and nitrosoproteome is presented characterising potential
carbonylated, nitrated and nitrosylated targets proteins with distinct or overlapped
signatures. This work provides a global framework to better understand the oxidative
and nitrosative signaling network under physiological and stressful conditions.
physiologic and pathologic conditions in plants, however knowledge on the oxidative
and nitrosative signaling outcomes is still unclear. To better understand how oxidative
and nitrosative signals are integrated to eventually regulate cellular adjustments to
external conditions, local and systemic responses were investigated in roots and
leaves of citrus plants after root treatment with H202 or sodium nitroprusside (SNP;
nitric oxide donor), pre-treatment with H202 or SNP followed by NaCI stress, and
direct NaCI stress for 8 days. Phenotypic and physiological data showed that preexposure
to H202 or SNP induced acclimation to subsequent salinity stress. Both
H202 and NO were locally and systemically accumulated in the citrus's tissues upon
chemical treatments and/or NaCl. Combined histochemical and fluorescent
approaches document the existence of a vascular tissue-driven long distance ROS and
NO signaling mechanism. Transcriptional analysis of genes diagnostic for H202 and
NO signaling just after chemical treatments or following 8 days of salinity revealed
various tissue- and time- specific feedbacklfeedforward mechanisms controlling
internal H202 and NO homeostasis. Also evidence presented showing that protein
carbonylation, nitration and S-nitrosylation encode an 'oxidative and nitrosative
memory' following by H202 or SNP pre-treatments that could be involved in
acclimation to salt stress. In addition, for the first time, a whole-plant analysis of the
oxyproteome, nitroproteome and nitrosoproteome is presented characterising potential
carbonylated, nitrated and nitrosylated targets proteins with distinct or overlapped
signatures. This work provides a global framework to better understand the oxidative
and nitrosative signaling network under physiological and stressful conditions.
File(s)![Thumbnail Image]()
Name
INPAS Limassol Meeting Abstract 2.pdf
Size
967.92 KB
Format
Adobe PDF
Checksum (MD5)
ac910a1361a74243b64a7c9e09a55cd0