Hydrogen sulfide confers systemic tolerance to salt and polyethylene glycol stress in strawberry plants
Journal
Molecular Approaches in Plant Abiotic Stress
Date Issued
July 5, 2011
Abstract
Hydrogen sulfide (H2S) is an endogenous gasotransmitter which has been recently found to play
a major signaling role in response to abiotic stress factors. In the present study we tested
whether hydroponic pre-treatment of strawberry (Fragaria x ananassa cv. Camarosa) roots to a
H2S donor, NaHS (10 mM for 48 h) could induce long lasting priming effects and tolerance to
subsequent exposure to 100 mM NaCI or 10% PEG-6000 for 7 d, employing a variety of
physiological, biochemical and molecular approaches. Hydrogen sulfide root pre-treatment
resulted in significantly increased leaf chlorophyll fluorescence, stomatal conductance and
relative leaf water content as well as reduced ion leakage and lipid peroxidation levels in
comparison with plants directly subjected to salt and PEG stress, suggesting a systemic
mitigating effect of H2S pre-treatment to cellular damage derived from abiotic stress factors. In
addition, root pre-treatment resulted in the minimization of oxidative and nitrosative stress in
strawberry plants, manifested via the reduced de novo synthesis of NO and H202 in leaves and
the maintenance of high ascorbate and glutathione redox states following subsequent salt and
hyperosmotic stresses. Quantitative real-time RT-PCR data examining antioxidant, transcription
factor and ion transporter gene expression levels suggest that H2S plays a key role in the
regulation of multiple transcriptional pathways. Our results indicate that H2S pre-treated plants
managed to overcome the deleterious effects of both salt and hyperosmotic stress, by
controlling oxidative and nitrosative damage mainly through increased performance of
antioxidative mechanism and thus propose a novel role of H2S in plant priming.
a major signaling role in response to abiotic stress factors. In the present study we tested
whether hydroponic pre-treatment of strawberry (Fragaria x ananassa cv. Camarosa) roots to a
H2S donor, NaHS (10 mM for 48 h) could induce long lasting priming effects and tolerance to
subsequent exposure to 100 mM NaCI or 10% PEG-6000 for 7 d, employing a variety of
physiological, biochemical and molecular approaches. Hydrogen sulfide root pre-treatment
resulted in significantly increased leaf chlorophyll fluorescence, stomatal conductance and
relative leaf water content as well as reduced ion leakage and lipid peroxidation levels in
comparison with plants directly subjected to salt and PEG stress, suggesting a systemic
mitigating effect of H2S pre-treatment to cellular damage derived from abiotic stress factors. In
addition, root pre-treatment resulted in the minimization of oxidative and nitrosative stress in
strawberry plants, manifested via the reduced de novo synthesis of NO and H202 in leaves and
the maintenance of high ascorbate and glutathione redox states following subsequent salt and
hyperosmotic stresses. Quantitative real-time RT-PCR data examining antioxidant, transcription
factor and ion transporter gene expression levels suggest that H2S plays a key role in the
regulation of multiple transcriptional pathways. Our results indicate that H2S pre-treated plants
managed to overcome the deleterious effects of both salt and hyperosmotic stress, by
controlling oxidative and nitrosative damage mainly through increased performance of
antioxidative mechanism and thus propose a novel role of H2S in plant priming.
File(s)![Thumbnail Image]()
Name
ROS Conference in Budapest 1.pdf
Size
1.13 MB
Format
Adobe PDF
Checksum (MD5)
7cc0020bf3389855287d3ded101e87c2