Sodium hydrosulfide induces systemic thermotolerance to strawberry plants through transcriptional regulation of heat shock proteins and aquaporin
Journal
BMC Plant Biology
Date Issued
February 5, 2014
DOI
10.1186/1471-2229-14-42
Abstract
Background: Temperature extremes represent an important limiting factor to plant growth and productivity. The
present study evaluated the effect of hydroponic pretreatment of strawberry (Fragaria x ananassa cv. ‘Camarosa’)
roots with an H2S donor, sodium hydrosulfide (NaHS; 100 μM for 48 h), on the response of plants to acute heat
shock treatment (42°C, 8 h).
Results: Heat stress-induced phenotypic damage was ameliorated in NaHS-pretreated plants, which managed to
preserve higher maximum photochemical PSII quantum yields than stressed plants. Apparent mitigating effects of
H2S pretreatment were registered regarding oxidative and nitrosative secondary stress, since malondialdehyde
(MDA), H2O2 and nitric oxide (NO) were quantified in lower amounts than in heat-stressed plants. In addition, NaHS
pretreatment preserved ascorbate/glutathione homeostasis, as evidenced by lower ASC and GSH pool redox
disturbances and enhanced transcription of ASC (GDH) and GSH biosynthetic enzymes (GS, GCS), 8 h after heat
stress imposition. Furthermore, NaHS root pretreatment resulted in induction of gene expression levels of an array
of protective molecules, such as enzymatic antioxidants (cAPX, CAT, MnSOD, GR), heat shock proteins (HSP70, HSP80,
HSP90) and aquaporins (PIP).
Conclusion: Overall, we propose that H2S root pretreatment activates a coordinated network of heat shock
defense-related pathways at a transcriptional level and systemically protects strawberry plants from heat
shock-induced damage.
present study evaluated the effect of hydroponic pretreatment of strawberry (Fragaria x ananassa cv. ‘Camarosa’)
roots with an H2S donor, sodium hydrosulfide (NaHS; 100 μM for 48 h), on the response of plants to acute heat
shock treatment (42°C, 8 h).
Results: Heat stress-induced phenotypic damage was ameliorated in NaHS-pretreated plants, which managed to
preserve higher maximum photochemical PSII quantum yields than stressed plants. Apparent mitigating effects of
H2S pretreatment were registered regarding oxidative and nitrosative secondary stress, since malondialdehyde
(MDA), H2O2 and nitric oxide (NO) were quantified in lower amounts than in heat-stressed plants. In addition, NaHS
pretreatment preserved ascorbate/glutathione homeostasis, as evidenced by lower ASC and GSH pool redox
disturbances and enhanced transcription of ASC (GDH) and GSH biosynthetic enzymes (GS, GCS), 8 h after heat
stress imposition. Furthermore, NaHS root pretreatment resulted in induction of gene expression levels of an array
of protective molecules, such as enzymatic antioxidants (cAPX, CAT, MnSOD, GR), heat shock proteins (HSP70, HSP80,
HSP90) and aquaporins (PIP).
Conclusion: Overall, we propose that H2S root pretreatment activates a coordinated network of heat shock
defense-related pathways at a transcriptional level and systemically protects strawberry plants from heat
shock-induced damage.
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