Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/3201
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Christou, Anastasis | - |
dc.contributor.author | Georgiadou, Egli | - |
dc.contributor.author | Filippou, Panagiota S. | - |
dc.contributor.author | Fotopoulos, Vasileios | - |
dc.contributor.author | Manganaris, George A. | - |
dc.contributor.other | Χρίστου, Αναστάσιος | - |
dc.contributor.other | Φιλίππου, Παναγιώτα | - |
dc.contributor.other | Φωτόπουλος, Βασίλειος | - |
dc.contributor.other | Μαγγανάρης, Γιώργος Α. | - |
dc.date.accessioned | 2015-03-20T12:02:09Z | - |
dc.date.accessioned | 2015-12-02T14:27:49Z | - |
dc.date.available | 2015-03-20T12:02:09Z | - |
dc.date.available | 2015-12-02T14:27:49Z | - |
dc.date.issued | 2014-03-01 | - |
dc.identifier.citation | Gene, 2014, vol. 537, no. 1, pp. 169-173 | en_US |
dc.identifier.issn | 03781119 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.14279/3201 | - |
dc.description.abstract | Strawberry plant tissues and particularly fruit material are rich in polysaccharides and polyphenolic compounds, thus rendering the isolation of nucleic acids a difficult task. This work describes the successful modification of a total RNA extraction protocol, which enables the isolation of high quantity and quality of total RNA from small amounts of strawberry leaf, root and fruit tissues. Reverse-transcription polymerase chain reaction (RT-PCR) amplification of GAPDH housekeeping gene from isolated RNA further supports the proposed protocol efficiency and its use for downstream molecular applications. This novel procedure was also successfully followed using other fruit tissues, such as olive and kiwifruit. In addition, optional treatment with RNase A following initial nucleic acid extraction can provide sufficient quality and quality of genomic DNA for subsequent PCR analyses, as evidenced from PCR amplification of housekeeping genes using extracted genomic DNA as template. Overall, this optimized protocol allows easy, rapid and economic isolation of high quality RNA from small amounts of an important fruit crop, such as strawberry, with extended applicability to other recalcitrant fruit crops. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Gene | en_US |
dc.rights | © Elsevier | en_US |
dc.subject | Genomic DNA extraction | en_US |
dc.subject | Fragaria × ananassa | en_US |
dc.subject | Polyphenols | en_US |
dc.subject | Polysaccharides | en_US |
dc.subject | Protocol | en_US |
dc.subject | Total RNA extraction | en_US |
dc.title | Establishment of a rapid, inexpensive protocol for extraction of high quality RNA from small amounts of strawberry plant tissues and other recalcitrant fruit crops | en_US |
dc.type | Article | en_US |
dc.collaboration | Cyprus University of Technology | en_US |
dc.collaboration | Agricultural Research Institute of Cyprus | en_US |
dc.subject.category | Agricultural Biotechnology | en_US |
dc.journals | Subscription | en_US |
dc.review | Peer Reviewed | en |
dc.country | Cyprus | en_US |
dc.subject.field | Agricultural Sciences | en_US |
dc.publication | Peer Reviewed | en_US |
dc.identifier.doi | 10.1016/j.gene.2013.11.066 | en_US |
dc.dept.handle | 123456789/70 | en |
dc.relation.issue | 1 | en_US |
dc.relation.volume | 537 | en_US |
cut.common.academicyear | 2013-2014 | en_US |
dc.identifier.spage | 169 | en_US |
dc.identifier.epage | 173 | en_US |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
crisitem.journal.journalissn | 0378-1119 | - |
crisitem.journal.publisher | Elsevier | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.orcid | 0000-0003-3974-988X | - |
crisitem.author.orcid | 0000-0003-1205-2070 | - |
crisitem.author.orcid | 0000-0002-5849-6104 | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
Appears in Collections: | Άρθρα/Articles |
CORE Recommender
SCOPUSTM
Citations
50
20
checked on Nov 9, 2023
WEB OF SCIENCETM
Citations
50
18
Last Week
0
0
Last month
0
0
checked on Oct 29, 2023
Page view(s) 50
365
Last Week
2
2
Last month
12
12
checked on May 12, 2024
Google ScholarTM
Check
Altmetric
Items in KTISIS are protected by copyright, with all rights reserved, unless otherwise indicated.