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Πεδίο DCΤιμήΓλώσσα
dc.contributor.authorChristou, Anastasis-
dc.contributor.authorGeorgiadou, Egli-
dc.contributor.authorFilippou, Panagiota S.-
dc.contributor.authorFotopoulos, Vasileios-
dc.contributor.authorManganaris, George A.-
dc.contributor.otherΧρίστου, Αναστάσιος-
dc.contributor.otherΦιλίππου, Παναγιώτα-
dc.contributor.otherΦωτόπουλος, Βασίλειος-
dc.contributor.otherΜαγγανάρης, Γιώργος Α.-
dc.date.accessioned2015-03-20T12:02:09Z-
dc.date.accessioned2015-12-02T14:27:49Z-
dc.date.available2015-03-20T12:02:09Z-
dc.date.available2015-12-02T14:27:49Z-
dc.date.issued2014-03-01-
dc.identifier.citationGene, 2014, vol. 537, no. 1, pp. 169-173en_US
dc.identifier.issn03781119-
dc.identifier.urihttps://hdl.handle.net/20.500.14279/3201-
dc.description.abstractStrawberry plant tissues and particularly fruit material are rich in polysaccharides and polyphenolic compounds, thus rendering the isolation of nucleic acids a difficult task. This work describes the successful modification of a total RNA extraction protocol, which enables the isolation of high quantity and quality of total RNA from small amounts of strawberry leaf, root and fruit tissues. Reverse-transcription polymerase chain reaction (RT-PCR) amplification of GAPDH housekeeping gene from isolated RNA further supports the proposed protocol efficiency and its use for downstream molecular applications. This novel procedure was also successfully followed using other fruit tissues, such as olive and kiwifruit. In addition, optional treatment with RNase A following initial nucleic acid extraction can provide sufficient quality and quality of genomic DNA for subsequent PCR analyses, as evidenced from PCR amplification of housekeeping genes using extracted genomic DNA as template. Overall, this optimized protocol allows easy, rapid and economic isolation of high quality RNA from small amounts of an important fruit crop, such as strawberry, with extended applicability to other recalcitrant fruit crops.en_US
dc.language.isoenen_US
dc.relation.ispartofGeneen_US
dc.rights© Elsevieren_US
dc.subjectGenomic DNA extractionen_US
dc.subjectFragaria × ananassaen_US
dc.subjectPolyphenolsen_US
dc.subjectPolysaccharidesen_US
dc.subjectProtocolen_US
dc.subjectTotal RNA extractionen_US
dc.titleEstablishment of a rapid, inexpensive protocol for extraction of high quality RNA from small amounts of strawberry plant tissues and other recalcitrant fruit cropsen_US
dc.typeArticleen_US
dc.collaborationCyprus University of Technologyen_US
dc.collaborationAgricultural Research Institute of Cyprusen_US
dc.subject.categoryAgricultural Biotechnologyen_US
dc.journalsSubscriptionen_US
dc.reviewPeer Revieweden
dc.countryCyprusen_US
dc.subject.fieldAgricultural Sciencesen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1016/j.gene.2013.11.066en_US
dc.dept.handle123456789/70en
dc.relation.issue1en_US
dc.relation.volume537en_US
cut.common.academicyear2013-2014en_US
dc.identifier.spage169en_US
dc.identifier.epage173en_US
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.journal.journalissn0378-1119-
crisitem.journal.publisherElsevier-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.orcid0000-0003-3974-988X-
crisitem.author.orcid0000-0003-1205-2070-
crisitem.author.orcid0000-0002-5849-6104-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
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