Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/20.500.14279/3162
Πεδίο DC | Τιμή | Γλώσσα |
---|---|---|
dc.contributor.author | Falara, Vasiliki | - |
dc.contributor.author | Fotopoulos, Vasileios | - |
dc.contributor.author | Margaritis, Thanasis | - |
dc.contributor.author | Anastasaki, Thalia | - |
dc.contributor.author | Pateraki, Irene | - |
dc.contributor.author | Bozabalidis, Artemios M. | - |
dc.contributor.author | Kafetzopoulos, Dimitris | - |
dc.contributor.author | Demetzos, Costas | - |
dc.contributor.author | Pichersky, Eran | - |
dc.contributor.author | Kanellis, Angelos K. | - |
dc.date.accessioned | 2009-05-26T10:25:26Z | en |
dc.date.accessioned | 2013-05-17T07:12:49Z | - |
dc.date.accessioned | 2015-12-02T14:26:31Z | - |
dc.date.available | 2009-05-26T10:25:26Z | en |
dc.date.available | 2013-05-17T07:12:49Z | - |
dc.date.available | 2015-12-02T14:26:31Z | - |
dc.date.issued | 2008-12 | - |
dc.identifier.citation | Plant Molecular Biology, 2008, vol. 68, iss. 6, pp. 633-651 | en_US |
dc.identifier.issn | 15735028 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.14279/3162 | - |
dc.description.abstract | Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from different developmental stages revealed that young leaves of 1–2 cm length displayed the highest content of labdane-type diterpenes (80 mg/g fresh weight) whereas trichomes from older leaves (2–3 or 3–4 cm) exhibited gradual decreased concentrations. A cDNA library was constructed enriched in transcripts from trichomes isolated from young leaves, which are characterized by high levels of labdane-type diterpenes. Functional annotation of 2,022 expressed sequence tags (ESTs) from the trichome cDNA library based on homology to A. thaliana genes suggested that 8% of the putative identified sequences were secondary metabolism-related and involved primarily in flavonoid and terpenoid biosynthesis. A significant proportion of the ESTs (38%) displayed no significant similarity to any other DNA deposited in databases, indicating a yet unknown function. Custom DNA microarrays constructed with 1,248 individual clones from the cDNA library facilitated transcriptome comparisons between trichomes and trichome-free tissues. In addition, gene expression studies in various Cistus tissues and organs for one of the genes highlighted as the most differentially expressed by the microarray experiments revealed a putative sesquiterpene synthase with a trichome-specific expression pattern. Full length cDNA isolation and heterologous expression in E. coli followed by biochemical analysis, led to the characterization of the produced protein as germacrene B synthase. | en_US |
dc.format | en_US | |
dc.language.iso | en | en_US |
dc.relation.ispartof | Plant Molecular Biology | en_US |
dc.rights | © Springer | en_US |
dc.subject | Cistus creticus subsp. creticus | en_US |
dc.subject | DNA microarrays | en_US |
dc.subject | EST analysis | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Germacrene B synthase | en_US |
dc.subject | Trichomes | en_US |
dc.title | Transcriptome analysis approaches for the isolation of trichome-specific genes from the medicinal plant Cistus creticus subsp. creticus | en_US |
dc.type | Article | en_US |
dc.collaboration | Aristotle University of Thessaloniki | en_US |
dc.collaboration | Cyprus University of Technology | en_US |
dc.collaboration | Foundation for Research & Technology-Hellas (F.O.R.T.H.) | en_US |
dc.collaboration | University of Crete | en_US |
dc.collaboration | National and Kapodistrian University of Athens | en_US |
dc.collaboration | Universitat de Barcelona | en_US |
dc.collaboration | University of Michigan | en_US |
dc.subject.category | Biological Sciences | en_US |
dc.journals | Subscription | en_US |
dc.review | peer reviewed | - |
dc.country | Greece | en_US |
dc.country | Cyprus | en_US |
dc.country | Spain | en_US |
dc.country | United States | en_US |
dc.subject.field | Natural Sciences | en_US |
dc.publication | Peer Reviewed | en_US |
dc.identifier.doi | 10.1007/s11103-008-9399-0 | en_US |
dc.dept.handle | 123456789/70 | en |
dc.relation.issue | 6 | en_US |
dc.relation.volume | 68 | en_US |
cut.common.academicyear | 2008-2009 | en_US |
dc.identifier.spage | 633 | en_US |
dc.identifier.epage | 651 | en_US |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
crisitem.journal.journalissn | 1573-5028 | - |
crisitem.journal.publisher | Springer Nature | - |
crisitem.author.dept | Department of Agricultural Sciences, Biotechnology and Food Science | - |
crisitem.author.faculty | Faculty of Geotechnical Sciences and Environmental Management | - |
crisitem.author.orcid | 0000-0003-1205-2070 | - |
crisitem.author.parentorg | Faculty of Geotechnical Sciences and Environmental Management | - |
Εμφανίζεται στις συλλογές: | Άρθρα/Articles |
CORE Recommender
SCOPUSTM
Citations
31
checked on 9 Νοε 2023
WEB OF SCIENCETM
Citations
50
29
Last Week
0
0
Last month
0
0
checked on 28 Οκτ 2023
Page view(s)
449
Last Week
2
2
Last month
28
28
checked on 13 Μαρ 2025
Google ScholarTM
Check
Altmetric
Όλα τα τεκμήρια του δικτυακού τόπου προστατεύονται από πνευματικά δικαιώματα