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Πεδίο DCΤιμήΓλώσσα
dc.contributor.authorFalara, Vasiliki-
dc.contributor.authorFotopoulos, Vasileios-
dc.contributor.authorMargaritis, Thanasis-
dc.contributor.authorAnastasaki, Thalia-
dc.contributor.authorPateraki, Irene-
dc.contributor.authorBozabalidis, Artemios M.-
dc.contributor.authorKafetzopoulos, Dimitris-
dc.contributor.authorDemetzos, Costas-
dc.contributor.authorPichersky, Eran-
dc.contributor.authorKanellis, Angelos K.-
dc.date.accessioned2009-05-26T10:25:26Zen
dc.date.accessioned2013-05-17T07:12:49Z-
dc.date.accessioned2015-12-02T14:26:31Z-
dc.date.available2009-05-26T10:25:26Zen
dc.date.available2013-05-17T07:12:49Z-
dc.date.available2015-12-02T14:26:31Z-
dc.date.issued2008-12-
dc.identifier.citationPlant Molecular Biology, 2008, vol. 68, iss. 6, pp. 633-651en_US
dc.identifier.issn15735028-
dc.identifier.urihttps://hdl.handle.net/20.500.14279/3162-
dc.description.abstractCistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from different developmental stages revealed that young leaves of 1–2 cm length displayed the highest content of labdane-type diterpenes (80 mg/g fresh weight) whereas trichomes from older leaves (2–3 or 3–4 cm) exhibited gradual decreased concentrations. A cDNA library was constructed enriched in transcripts from trichomes isolated from young leaves, which are characterized by high levels of labdane-type diterpenes. Functional annotation of 2,022 expressed sequence tags (ESTs) from the trichome cDNA library based on homology to A. thaliana genes suggested that 8% of the putative identified sequences were secondary metabolism-related and involved primarily in flavonoid and terpenoid biosynthesis. A significant proportion of the ESTs (38%) displayed no significant similarity to any other DNA deposited in databases, indicating a yet unknown function. Custom DNA microarrays constructed with 1,248 individual clones from the cDNA library facilitated transcriptome comparisons between trichomes and trichome-free tissues. In addition, gene expression studies in various Cistus tissues and organs for one of the genes highlighted as the most differentially expressed by the microarray experiments revealed a putative sesquiterpene synthase with a trichome-specific expression pattern. Full length cDNA isolation and heterologous expression in E. coli followed by biochemical analysis, led to the characterization of the produced protein as germacrene B synthase.en_US
dc.formatpdfen_US
dc.language.isoenen_US
dc.relation.ispartofPlant Molecular Biologyen_US
dc.rights© Springeren_US
dc.subjectCistus creticus subsp. creticusen_US
dc.subjectDNA microarraysen_US
dc.subjectEST analysisen_US
dc.subjectGene expressionen_US
dc.subjectGermacrene B synthaseen_US
dc.subjectTrichomesen_US
dc.titleTranscriptome analysis approaches for the isolation of trichome-specific genes from the medicinal plant Cistus creticus subsp. creticusen_US
dc.typeArticleen_US
dc.collaborationAristotle University of Thessalonikien_US
dc.collaborationCyprus University of Technologyen_US
dc.collaborationFoundation for Research & Technology-Hellas (F.O.R.T.H.)en_US
dc.collaborationUniversity of Creteen_US
dc.collaborationNational and Kapodistrian University of Athensen_US
dc.collaborationUniversitat de Barcelonaen_US
dc.collaborationUniversity of Michiganen_US
dc.subject.categoryBiological Sciencesen_US
dc.journalsSubscriptionen_US
dc.reviewpeer reviewed-
dc.countryGreeceen_US
dc.countryCyprusen_US
dc.countrySpainen_US
dc.countryUnited Statesen_US
dc.subject.fieldNatural Sciencesen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1007/s11103-008-9399-0en_US
dc.dept.handle123456789/70en
dc.relation.issue6en_US
dc.relation.volume68en_US
cut.common.academicyear2008-2009en_US
dc.identifier.spage633en_US
dc.identifier.epage651en_US
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.journal.journalissn1573-5028-
crisitem.journal.publisherSpringer Nature-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.orcid0000-0003-1205-2070-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
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