Please use this identifier to cite or link to this item:
Title: Biomarkers of oxidative stress in urine and plasma of operators at six Singapore printing centers and their association with several metrics of printer-emitted nanoparticle exposures
Authors: Bello, Dhimiter 
Chanetsa, Lucia 
Christophi, Costas A. 
Singh, Dilpreet 
Setyawati, Magdiel Inggrid 
Christiani, David C. 
Chotirmall, Sanjay H. 
Ng, Kee Woei 
Demokritou, Philip 
Major Field of Science: Medical and Health Sciences
Field Category: Health Sciences
Keywords: 8OHdG;DNA damage;engineered nanoparticles;isoprostane;Laser printers and photocopiers;malondialdehyde;oxidative stress;plasma;total protein carbonyl;urine
Issue Date: 1-Jan-2022
Source: Nanotoxicology, 2022, vol.16, iss. 9-10, pp. 913 - 934
Volume: 16
Issue: 9-10
Start page: 913
End page: 934
Abstract: Inhalation of nanoparticles emitted from toner-based printing equipment (TPE), such as laser printers and photocopiers, also known as PEPs, has been associated with systemic inflammation, hypertension, cardiovascular disease, respiratory disorders, and genotoxicity. Global serum metabolomics analysis in 19 healthy TPE operators found 52 dysregulated biomolecules involved in upregulation of inflammation, immune, and antioxidant responses and downregulation of cellular energetics and cell proliferation. Here, we build on the metabolomics study by investigating the association of a panel of nine urinary OS biomarkers reflecting DNA/RNA damage (8OHdG, 8OHG, and 5OHMeU), protein/amino acid oxidation (o-tyrosine, 3-chlorotyrosine, and 3-nitrotyrosine), and lipid oxidation (8-isoprostane, 4-hydroxy nonenal, and malondialdehyde [MDA]), as well as plasma total MDA and total protein carbonyl (TPC), with several nanoparticle exposure metrics in the same 19 healthy TPE operators. Plasma total MDA, urinary 5OHMeU, 3-chlorotyrosine, and 3-nitrotyrosine were positively, whereas o-tyrosine inversely and statistically significantly associated with PEPs exposure in multivariate models, after adjusting for age and urinary creatinine. Urinary 8OHdG, 8OHG, 5OHMeU, and total MDA in urine and plasma had group mean values higher than expected in healthy controls without PEPs exposure and comparable to those of workers experiencing low to moderate levels of oxidative stress (OS). The highest exposure group had OS biomarker values, most notably 8OHdG, 8OHG, and total MDA, that compared to workers exposed to welding fumes and titanium dioxide. Particle number concentration was the most sensitive and robust exposure metric. A combination of nanoparticle number concentration and OS potential of fresh aerosols is recommended for larger scale future studies.
ISSN: 17435390
DOI: 10.1080/17435390.2023.2175735
Rights: © Elsevier B.V.
Attribution-NonCommercial-NoDerivatives 4.0 International
Type: Article
Affiliation : University of Massachusetts Lowell 
Harvard T.H. Chan School of Public Health 
Cyprus University of Technology 
Nanyang Technological University 
Massachusetts General Hospital/Harvard Medical School 
Tan Tock Seng Hospital 
Appears in Collections:Άρθρα/Articles

CORE Recommender
Show full item record

Page view(s)

Last Week
Last month
checked on Dec 9, 2023

Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons