Photoreduction of carotenoids in the aerobic anoxygenic photoheterotrophs probed by real time Raman spectroscopy

Abstract

The Aerobic anoxygenic phototrophic bacteria (AAPB) Roseobacter denitrificans and Roseobacter litoralis are widespread in the bacterioplankton community with a particular role in the marine carbon cycle. Measurements of carotenoids isolated from dark-grown cells indicated the presence of spheroidenone (SO, N = 11) and of 3,4 dihydrospheroidenone (N = 10) in the carotenoids isolated from illuminated cells. Time-dependent Raman 514 nm excitation experiments of R. denitrificans and R. litoralis cells grown under illumination demonstrated that v1 (C=C) of SO exhibits a time-dependent substantial frequency upshift relative to its frequency in the dark-grown cells, in a manner resembling shorting the conjugation length (N). We suggest that the irreversible dark-SO to light- 3,4 dihydrospheroidenone transition observed in the intact carotenoids of R. denitrificans and R. litoralis cells is an operative photoreduction strategy of SO containing AAPB that affects the energy transfer mechanism.