Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14279/1921
Title: Detection of a photostable five-coordinate heme a3-Fe-Co species and functional implications of His384/α10 in CO-bound ba 3-cytochrome c oxidase from Thermus thermophilus
Authors: Pinakoulaki, Eftychia 
Ohta, Takehiro 
Varotsis, Constantinos 
metadata.dc.contributor.other: Πινακουλάκη, Ευτυχία
Βαρώτσης, Κωνσταντίνος
Major Field of Science: Engineering and Technology
Field Category: Chemical Engineering
Keywords: Stereochemistry;Cadmium compounds;Carbon monoxide;Copper;Enzymes;Iron compounds;Mercury compounds;Molecules;Spectroscopy;Quartz
Issue Date: 10-Apr-2004
Source: Journal of physical chemistry B, 2004, vol. 108, no. 18, pp. 5489-5491
Volume: 108
Issue: 18
Start page: 5489
End page: 5491
Journal: Journal of Physical Chemistry B 
Abstract: Resonance Raman (RR) spectra are reported for the fully reduced carbon monoxy derivative of ba3-cytochrome c oxidase from Thermus thermophilus. The RR spectra show the formation of a photolabile six-coordinate heme-CO and a photostable five-coordinate heme Fe-CO species. The latter species is formed by the cleavage of the proximal heme Fe-His384 bond and is the first five-coordinate Fe-CO species detected in heme-copper oxidases. The frequency of the Fe-CO species observed at 526 cm-1 correlates with either the C-O stretching modes observed at 1967 or 1982 cm-1 and lie on the correlation line of v(Fe-CO) vs v(C-O) for all known five-coordinate heme Fe-CO complexes. The loss of intensity of the heme Fe-His384 mode observed at 193 cm-1 in the photostationary CO-bound spectra is attributed to the loss of the non-hydrogen bonded heme Fe-His384⋯Gly359 conformer. Taken together, the data indicate that the environment of the ruptured His384 that is a part of the Q-proton pathway and leads to the highly conserved among all heme-copper oxidases, H2O pool, is disrupted upon CO binding to heme a3
URI: https://hdl.handle.net/20.500.14279/1921
ISSN: 15206106
DOI: 10.1021/jp049259k
Rights: © American Chemical Society
Attribution-NonCommercial-NoDerivs 3.0 United States
Type: Article
Affiliation: University of Crete 
Affiliation : University of Cyprus 
Publication Type: Peer Reviewed
Appears in Collections:Άρθρα/Articles

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