Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14279/19049
Title: | Probing hemoglobin glyco-products by fluorescence spectroscopy |
Authors: | Ioannou, Aristos Varotsis, Constantinos |
Major Field of Science: | Natural Sciences |
Field Category: | Chemical Sciences |
Keywords: | Amino acids;Fluorescence;Fluorescence spectroscopy;Glycosylation;Proteins;Reaction intermediates;Spectroscopic analysis |
Issue Date: | 19-Nov-2019 |
Source: | RSC Advances, 2019, vol. 9, no. 64, pp. 37614-37619 |
Volume: | 9 |
Issue: | 64 |
Start page: | 37614 |
End page: | 37619 |
Journal: | RSC Advances |
Abstract: | Maillard reaction products (MRPs) participate in reactions of carbohydrate intermediates with proteins, resulting in the formation of advanced glycation end-products (AGEs). Dietary Maillard reaction products are recognized as potential chemical modifiers of human proteins. We have investigated the reaction of isolated MRPs from an asparagine-glucose model system with hemoglobin (Hb) to elucidate the binding effect of the MRPs in hemoglobin by fluorescence spectrophotometry. The tryptophan-specific fluorescence obtained for glycated hemoglobin exhibited a Stokes effect since the wavelength of the emission peak was shifted to a higher wavelength than that of native Hb. The formation of new fluorescence emission features indicates the formation of modified hemoglobin species. Fluorescence spectroscopic studies provide evidence that the conformational changes in the β-Trp 37 moiety induce motion of the distal His 64 (E7) in the heme binding pocket. This results in the formation of inactive hemichrome forms of hemoglobin which are related to blood disorders. |
URI: | https://hdl.handle.net/20.500.14279/19049 |
ISSN: | 20462069 |
DOI: | 10.1039/C9RA05243G |
Rights: | © Royal Society of Chemistry Attribution-NonCommercial-NoDerivatives 4.0 International |
Type: | Article |
Affiliation : | Cyprus University of Technology |
Publication Type: | Peer Reviewed |
Appears in Collections: | Άρθρα/Articles |
Files in This Item:
File | Description | Size | Format | |
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c9ra05243g.pdf | Fulltext | 864.09 kB | Adobe PDF | View/Open |
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