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Πεδίο DCΤιμήΓλώσσα
dc.contributor.authorManganaris, George A.-
dc.contributor.authorEconomou, Athanasios Sotirios-
dc.contributor.authorBoubourakas, I.N.-
dc.contributor.authorKatis, Nikolaos I.-
dc.date.accessioned2009-11-27T06:52:59Zen
dc.date.accessioned2013-05-16T06:25:25Z-
dc.date.accessioned2015-12-02T09:54:27Z-
dc.date.available2009-11-27T06:52:59Zen
dc.date.available2013-05-16T06:25:25Z-
dc.date.available2015-12-02T09:54:27Z-
dc.date.issued2003-10-
dc.identifier.citationPlant Cell Reports, Volume 22, Issue 3, October 2003, Pages 195-200en_US
dc.identifier.issn07217714-
dc.identifier.issn1432203X-
dc.identifier.urihttps://hdl.handle.net/20.500.14279/1751-
dc.description.abstractThe plum pox virus (PPV) and prunus necrotic ringspot virus (PNRSV) cause serious disease problems in stone-fruit trees. In this work, the possibility of obtaining plant material free from these viruses through thermotherapy and meristem-tip culture from infected nectarine shoots (Prunus persica var. nectarina Max, cv. ''Arm King'') was studied. In addition, the detection of these viruses in in vitro cultures and young acclimatized plantlets with double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) was studied. Meristem-tip explants (0.8–1.3 mm) derived from sprouted buds of winter wood and spring shoots from field grown plants had a 2–5% regeneration response. However, application of thermotherapy to potted nectarine trees (3 weeks at a maximum temperature of 35°C) facilitated excision of longer meristem tips (1.3–2.0 mm) that resulted in a significantly higher regeneration response (38%) in woody plant medium (WPM) without plant growth regulators. Such explants formed multiple shoots with the addition of 8 M benzylaminopurine and 0.8 M indoleacetic acid. When they were tested for the presence of PPV and PNRSV, 86% and 81% were found to be virus-free as detected by DAS-ELISA and multiplex RT-PCR, respectively. Individual shoots excised from virus-free cultures readily rooted in vitro (half-strength WPM plus 2 M indolebutyric acid) and grew to plantlets. The combination of an efficient protocol for virus elimination and the establishment of highly sensitive diagnostics resulted in the production of nectarine plants free from PPV and PNRSV.en_US
dc.formatpdfen_US
dc.language.isoenen_US
dc.relation.ispartofPlant Cell Reportsen_US
dc.rights© 2003 Springer-Verlagen_US
dc.subjectMeristem cultureen_US
dc.subjectPlum pox virusen_US
dc.subjectPrunus necrotic ringspot virusen_US
dc.subjectPrunus persicaen_US
dc.subjectVirusen_US
dc.subjectIndexeden_US
dc.titleElimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarineen_US
dc.typeArticleen_US
dc.collaborationAristotle University of Thessalonikien_US
dc.collaborationInternational Hellenic Universityen_US
dc.subject.categoryAGRICULTURAL SCIENCESen_US
dc.subject.categoryAgricultural Biotechnologyen_US
dc.subject.categoryOther Agricultural Sciencesen_US
dc.countryGreeceen_US
dc.subject.fieldAgricultural Sciencesen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1007/s00299-003-0681-yen_US
dc.dept.handle123456789/54en
cut.common.academicyear2019-2020en_US
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.journal.journalissn1432-203X-
crisitem.journal.publisherSpringer Nature-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.orcid0000-0002-5849-6104-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
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