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dc.contributor.authorKapari-Isaia, T.-
dc.contributor.authorKyriakou, A. P.-
dc.contributor.authorPapayiannis, Lambros C.-
dc.contributor.authorGregoriou, S.-
dc.contributor.authorPsaltis, I.-
dc.contributor.authorTsaltas, Dimitrios-
dc.contributor.otherΤσάλτας, Δημήτρης-
dc.date.accessioned2012-05-11T05:35:32Zen
dc.date.accessioned2013-05-17T07:12:48Z-
dc.date.accessioned2015-12-02T08:38:48Z-
dc.date.available2012-05-11T05:35:32Zen
dc.date.available2013-05-17T07:12:48Z-
dc.date.available2015-12-02T08:38:48Z-
dc.date.issued2008-04-
dc.identifier.citationPlant Pathology, 2008, Volume 57, Issue 2, Pages 348-353.en_US
dc.identifier.issn00320862-
dc.identifier.urihttps://hdl.handle.net/20.500.14279/1018-
dc.description.abstractA new laboratory technique combining shoot-tip grafting in vitro and biological indexing on indicator plants was explored for the detection of citrus exocortis and related viroids. Three in vitro laboratory methods were used and compared with the classical biological method. With the classical in vivo method, diagnosis is based on the expression of symptoms on indicators 11-14 weeks after inoculation. In contrast, with the first in vitro method, microindexing in vitro of citron seedlings by graft inoculation, diagnosis was possible 12 days after inoculation; with the second method, microindexing in vitro of citron cuttings by graft inoculation, 20 days after inoculation; and with the third method, microindexing in vitro of citron cuttings by injection inoculation, 40 days after inoculation. Inoculated Etrog citron plantlets grown in vitro and tested by RT-PCR showed the same viroid content as the source plants. Of the three in vitro viroid indexing methods, microindexing on cuttings by grafting was easier and more reliable than microindexing either on seedlings or on cuttings by injection.en_US
dc.language.isoenen_US
dc.subjectCitrus exocortis diseaseen_US
dc.subjectCitrus spp.en_US
dc.subjectDetection and diagnosisen_US
dc.subjectEtrog citronen_US
dc.titleRapid in vitro microindexing of viroids in citrusen_US
dc.typeArticleen_US
dc.collaborationMiddlesex Universityen_US
dc.collaborationAgricultural Research Institute of Cyprusen_US
dc.subject.categoryEnvironmental Biotechnologyen_US
dc.subject.categoryAGRICULTURAL SCIENCESen_US
dc.subject.categoryOther Agricultural Sciencesen_US
dc.countryCyprusen_US
dc.countryUnited Kingdomen_US
dc.subject.fieldAgricultural Sciencesen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1111/j.1365-3059.2007.01774.xen_US
dc.dept.handle123456789/54en
cut.common.academicyear2007-2008en_US
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.orcid0000-0001-6546-3602-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
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