Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14279/10063
Title: Short communication: Identification of variation in the ovine prolactin gene of Chios sheep with a cost-effective sequence-based typing assay
Authors: Miltiadou, Despoina 
Orford, Michael R. 
Symeou, Simoni 
Banos, Georgios 
Major Field of Science: Agricultural Sciences
Field Category: Agricultural Biotechnology
Keywords: SNP identification;Prolactin association;Sequence-based typing (SBT);Sheep
Issue Date: 1-Feb-2017
Source: Journal of Dairy Science, 2017, vol. 100, no. 2, pp. 1290-1294
Volume: 100
Issue: 2
Start page: 1290
End page: 1294
Journal: Journal of Dairy Science 
Abstract: The present study identified single nucleotide polymorphisms (SNP) in the coding and untranslated regions of the ovine prolactin gene of Chios sheep. By developing a cost-effective direct sequence-based typing assay, around 600 bp of reliable sequencing data and clear identification of heterozygous positions was achieved. Five SNP were found, located in exons 2 (KC764410:g.567G>A, g.625C>T, g.683C>A) and 3 (KC764410:g.2015C>A, g.2101G>A), whereas the remaining exons were monomorphic. The identified SNP were synonymous, with the exception of the g.567G>A SNP, which results in an Arg to His amino acid change. As the sequencing cost of the sequence-based typing assay was 20 orders of magnitude lower compared with a standard Sanger method, the assay was also used as a genotyping tool. The identified polymorphism was genotyped for 247 ewes and was subsequently used in mixed model association analyses of milk yield, milk fat content, and litter size at birth. The association analysis revealed a significant dominance effect of 0.17 ± 0.07 of the g.2015C>A SNP on milk fat percentage, whereas a dominance effect of −21.33 ± 10.51 of the same SNP on total lactation milk yield was also estimated. The g.2015C>A SNP explained 2.47 and 3.68% of the total phenotypic variance of milk yield and milk fat percentage, respectively, whereas the corresponding values for the animal variance were 7.14 and 11.75%. A suggestive association of the nonsynonymous g.567G>A SNP with litter size at birth was also detected.
URI: https://hdl.handle.net/20.500.14279/10063
ISSN: 00220302
DOI: 10.3168/jds.2016-11721
Rights: © American Dairy Science Association
Type: Article
Affiliation : Cyprus University of Technology 
The University of Edinburgh 
Publication Type: Peer Reviewed
Appears in Collections:Άρθρα/Articles

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