Διαχωρισμός και ανάκτηση πρωτεϊνών και σακχάρων από τυρόγαλο χαλλουμιού με τη χρήση μεμβρανών υπερδιήθησης
Date Issued
2012
Author(s)
Advisor
Abstract
The current thesis investigated the separation and recovery of proteins and sugars from halloumi
whey. During the process of halloumi manufacturing, big volumes of whey is produced as waste.
The whey is high in organic matter and its processing is a costly process.
The objective of the current study was to investigate the separation and the recovery of high
added value components (proteins and sugars) as byproducts of halloumi whey. For this purpose,
two samples of industrially produced whey were processed using five types of ultrafiltration
membranes with different Molecular Weight Cut Offs (MWCO: 100, 50, 20, 2, 1 kDa) under
various transmembrane pressures. The experiments were performed in a laboratory size
membrane apparatus (Alfa Laval Labunit M10).
In the first part of the experiment, ultrafiltration was conducted using three types of membranes
cutoff 100, 50 and 20 kDa. Two replicates were performed for each sample and each type of
membrane. Retention coefficients and performance parameters were monitored for each
experiment. Feed and permeate was collected for analysis.
In the second part of the experimental procedure permeate from the 20 kDa membrane was used
as feed for the membranes treated with 2 and 1 kDa, and similar procedures as the first part were
followed. Performance parameters and coefficients were studied for each experimental
combination. Analyses were made for retention of protein, total sugars and total phenols.
According to the results, all the membranes had a high retention rate of protein (70-90%). The
disadvantages of membranes with smaller MWCO were the relatively high retention of total
sugars in the concentrate (20-40%) and the low flux. However, the optimum separation was
performed using the 100 kDa membrane which led to ~ 75% of proteins remaining in the
concentrate, while ~ 98% of total sugars passed in the filtrate achieving the separation of two
components. The optimum operating parameters were at a pressure of 3 bar where the flux was ~
12 Lm -2h-1 and the relative flux was around 50%.
whey. During the process of halloumi manufacturing, big volumes of whey is produced as waste.
The whey is high in organic matter and its processing is a costly process.
The objective of the current study was to investigate the separation and the recovery of high
added value components (proteins and sugars) as byproducts of halloumi whey. For this purpose,
two samples of industrially produced whey were processed using five types of ultrafiltration
membranes with different Molecular Weight Cut Offs (MWCO: 100, 50, 20, 2, 1 kDa) under
various transmembrane pressures. The experiments were performed in a laboratory size
membrane apparatus (Alfa Laval Labunit M10).
In the first part of the experiment, ultrafiltration was conducted using three types of membranes
cutoff 100, 50 and 20 kDa. Two replicates were performed for each sample and each type of
membrane. Retention coefficients and performance parameters were monitored for each
experiment. Feed and permeate was collected for analysis.
In the second part of the experimental procedure permeate from the 20 kDa membrane was used
as feed for the membranes treated with 2 and 1 kDa, and similar procedures as the first part were
followed. Performance parameters and coefficients were studied for each experimental
combination. Analyses were made for retention of protein, total sugars and total phenols.
According to the results, all the membranes had a high retention rate of protein (70-90%). The
disadvantages of membranes with smaller MWCO were the relatively high retention of total
sugars in the concentrate (20-40%) and the low flux. However, the optimum separation was
performed using the 100 kDa membrane which led to ~ 75% of proteins remaining in the
concentrate, while ~ 98% of total sugars passed in the filtrate achieving the separation of two
components. The optimum operating parameters were at a pressure of 3 bar where the flux was ~
12 Lm -2h-1 and the relative flux was around 50%.
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