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|Title:||Detection of Mycobacterium avium subsp. paratuberculosis in bulk tank milk by combined phage-PCR assay: Evidence that plaque number is a good predictor of MAP||Authors:||Botsaris, George
Dodd, Christine E R
Rees, Catherine E.D.
|Keywords:||Detection;Milk;Paratuberculosis;Phage-PCR assay||Category:||Other Engineering and Technologies||Field:||Engineering and Technology||Issue Date:||3-Jun-2013||Publisher:||Elsevier||Source:||International Journal of Food Microbiology, 2013, Volume 164, Issue 1, Pages 76-80||DOI:||http://dx.doi.org/10.1016/j.ijfoodmicro.2013.03.023||Abstract:||Conventional culture and a rapid phage-PCR method were used to detect Mycobacterium avium subsp. paratuberculosis (MAP) in bulk tank milk (BTM) samples. Only two of 225 samples (0.9%) were found to contain MAP by culture whereas 50 (22%) MAP-positive samples were identified using the phage-PCR assay, including both samples that were MAP-culture positive. Results using the phage-based method for independently tested duplicate samples indicated that the assay is very reproducible (r2=0.897), especially when low levels of mycobacteria are present. A relationship was established between plaque number and the presence of MAP in a sample. A cut-off value was determined allowing identification of MAP-positive samples based on plaque number alone (90% sensitivity, 99% specificity; area under the curve=0.976). These results indicate that the assay is a robust method for screening BTM, providing results within 24h.||URI:||http://ktisis.cut.ac.cy/handle/10488/9851||ISSN:||01681605||Rights:||© 2013 Elsevier B.V.||Type:||Article|
|Appears in Collections:||Άρθρα/Articles|
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