Please use this identifier to cite or link to this item:
Title: Detection of Mycobacterium avium subsp. paratuberculosis in bulk tank milk by combined phage-PCR assay: Evidence that plaque number is a good predictor of MAP
Authors: Botsaris, George 
Liapi, Maria 
Kakogiannis, Charalambos 
Dodd, Christine E R 
Rees, Catherine E.D. 
Keywords: Detection;Milk;Paratuberculosis;Phage-PCR assay
Category: Other Engineering and Technologies
Field: Engineering and Technology
Issue Date: 3-Jun-2013
Publisher: Elsevier
Source: International Journal of Food Microbiology, 2013, Volume 164, Issue 1, Pages 76-80
Abstract: Conventional culture and a rapid phage-PCR method were used to detect Mycobacterium avium subsp. paratuberculosis (MAP) in bulk tank milk (BTM) samples. Only two of 225 samples (0.9%) were found to contain MAP by culture whereas 50 (22%) MAP-positive samples were identified using the phage-PCR assay, including both samples that were MAP-culture positive. Results using the phage-based method for independently tested duplicate samples indicated that the assay is very reproducible (r2=0.897), especially when low levels of mycobacteria are present. A relationship was established between plaque number and the presence of MAP in a sample. A cut-off value was determined allowing identification of MAP-positive samples based on plaque number alone (90% sensitivity, 99% specificity; area under the curve=0.976). These results indicate that the assay is a robust method for screening BTM, providing results within 24h.
ISSN: 01681605
Rights: © 2013 Elsevier B.V.
Type: Article
Appears in Collections:Άρθρα/Articles

Show full item record

Page view(s)

Last Week
Last month
checked on Jun 12, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.