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Title: HPLC-SPE-NMR characterization of major metabolites in Salvia fruticosa mill. Extract with antifungal potential: Relevance of carnosic acid, carnosol, and hispidulin
Authors: Exarchou, Vassiliki 
Kanetis, Loukas 
Charalambous, Zenovia 
Apers, Sandra 
Pieters, Luc A C 
Gekas, Vassilis 
Goulas, Vlasios 
Keywords: Aspergillus tubingensis;Botrytis cinerea;Greek sage;Penicillium digitatum;diterpenes;flavonoids;phytochemicals;q-NMR
Category: Agricultural Biotechnology
Field: Agricultural Sciences
Issue Date: 21-Jan-2015
Publisher: American Chemical Society
Source: Journal of Agricultural and Food Chemistry, Volume 63, Issue 2, 21 January 2015, Pages 457-463
DOI: 10.1021/jf5050734
Journal: Journal of Agricultural and Food Chemistry 
Abstract: Plant pathogenic fungi are considered of significant economic importance for adversely affecting both quantitatively and qualitatively fresh and processed produce. Extracts of Salvia fruticosa were initially screened for their antifungal activity, and the ethyl acetate fraction, being the most active, was further analyzed using HPLC-SPE-NMR hyphenation. The methoxylated flavones hispidulin, salvigenin, and cirsimaritin and the diterpenes carnosic acid, carnosol, and 12-methoxycarnosic acid were identified as the major components of the extract. In addition, the concentration levels of all identified components were determined using q-NMR. The antifungal activity of the crude extract and selected phytochemicals was estimated against the fungal species Aspergillus tubingensis, Botrytis cinerea, and Penicillium digitatum. The estimated MIC and MFC values of the ethyl acetate extract of S. fruticosa, as well as three of its major constituents, carnosic acid, carnosol, and hispidulin, support their antifungal activity, especially against B. cinerea and P. digitatum, suggesting their potential use in food and agricultural systems.
ISSN: 00218561
Rights: © 2014 American Chemical Society.
Type: Article
Appears in Collections:Άρθρα/Articles

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