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Title: Edible coating composed of chitosan and Salvia fruticosa Mill. extract for the control of grey mould of table grapes
Authors: Kanetis, Loukas 
Exarchou, Vassiliki 
Charalambous, Zinovia 
Goulas, Vlasios 
Keywords: Botrytis cinerea;Salvia fruticosa;Chitosan;Edible coating;Postharvest;Table grapes
Category: Agricultural Biotechnology
Field: Agricultural Sciences
Issue Date: 30-Jan-2017
Publisher: John Wiley and Sons Ltd
Source: Journal of the Science of Food and Agriculture, 2017, Volume 97, Issue 2, Pages 452-460
DOI: 10.1002/jsfa.7745
Journal: Journal of the Science of Food and Agriculture 
Abstract: BACKGROUND:Consumer concerns regarding high-quality produce, free of pesticide residues, direct research towards disease management strategies that minimise or even exclude the use of synthetic chemistries in crop production. The efficacy of a chitosan-based edible coating combined with the acetonic extract of Salvia fruticosa Mill. (ASF) was assessed against the grey mould of table grapes. RESULTS: HPLC-SPE-NMR and q-NMR analyses defined major constituents of ASF to be the flavonoids hispidulin, salvigenin and cirsimaritin and the diterpenes carnosic acid, carnosol and the 12-methoxycarnosic acid. The extract was found to be efficacious in reducing spore germination and mycelial growth of Botrytis cinerea in vitro at 10 and 25 °C. However, the combination of the ASF with chitosan 1% (w/v; CHIT) significantly improved fungal inhibition. Similarly, in fruit inoculation trials at 10 °C, the efficacy of the combined application of the ASF at 500 mg L−1 with CHIT against grey mould was statistically equal to the synthetic fungicide thiabendazole, ranging from 98.4% to 92.7% at 12 and 21 days post-inoculation, respectively. Furthermore, chitosan coating alone and in combination with ASF decreased the rate of fruit weight loss during cold storage, while preserved soluble solids content and titratable acidity. Chitosan-based coatings did not affect quality attributes and the bioactive compounds in table grapes. CONCLUSION: The combined application of the ASF in the form of an edible coating with chitosan could effectively control B. cinerea without deteriorating quality and physico-chemical properties of grapes. © 2016 Society of Chemical Industry.
ISSN: 00225142
Rights: © 2016 Society of Chemical Industry
Type: Article
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