Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/9204
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dc.contributor.authorTsipa, Argyro-
dc.contributor.authorKoutinas, Michalis-
dc.contributor.authorPistikopoulos, Efstratios N.-
dc.contributor.authorMantalaris, Athanasios A.-
dc.contributor.otherΚουτίνας, Μιχάλης-
dc.date.accessioned2017-01-23T12:50:24Z-
dc.date.available2017-01-23T12:50:24Z-
dc.date.issued2016-06-20-
dc.identifier.citationJournal of Biotechnology, 2016, Volume 228, Pages 112-123en_US
dc.identifier.issn01681656-
dc.identifier.urihttp://ktisis.cut.ac.cy/handle/10488/9204-
dc.description.abstractThe TOL plasmid promoters are activated by toluene leading to gene expression responsible for the degradation of the environmental signal. Benzoate is formed as an intermediate, activating the BenR protein of the chromosomal ortho-cleavage pathway that up-regulates the chromosomal PbenA promoter and the TOL Pm promoter resulting in cross-talk between the two networks. Herein, the transcriptional kinetics of the PbenR and PbenA promoters in conjunction with TOL promoters was monitored by real-time PCR during toluene biodegradation of different concentrations in batch cultures. The cross-talk between the two pathways was indicated by the simultaneous maximal expression of the Pm and PbenR promoters, as well as the transcriptional activation from PbenA occurring prior to PbenR, which indicates the potential up-regulation of PbenA by the TOL XylS protein. The repressory effect of toluene on Pr was evident for concentrations higher than 0.3 mM suggesting a threshold value for restoring the promoter's activity, while all the other promoters followed a specific expression pattern, regardless of the initial inducer concentration. Induction of the system with higher toluene concentrations revealed an oscillatory behaviour of Pm, the expression of which remained at high levels until the late exponential phase, demonstrating a novel function of this network.en_US
dc.formatpdfen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© Copyright 2016 Elsevier B.V.en_US
dc.subjectGene regulationen_US
dc.subjectPseudomomas putida mt-2en_US
dc.subjectTOL plasmiden_US
dc.subjectTolueneen_US
dc.subjectOrtho-cleavage pathwayen_US
dc.subjectTranscriptional kineticsen_US
dc.titleTranscriptional kinetics of the cross-talk between the ortho-cleavage and TOL pathways of toluene biodegradation in Pseudomonas putida mt-2en_US
dc.typeArticleen_US
dc.collaborationCyprus University of Technologyen_US
dc.collaborationImperial College Londonen_US
dc.collaborationUniversity of Texasen_US
dc.subject.categoryEarth and Related Environmental Sciencesen_US
dc.journalsSubscription Journalen_US
dc.countryUnited Kingdomen_US
dc.countryCyprusen_US
dc.countryUnited Statesen_US
dc.subject.fieldNatural Sciencesen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1016/j.jbiotec.2016.03.053-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.languageiso639-1other-
crisitem.author.deptDepartment of Environmental Science and Technology-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.orcid0000-0002-5371-4280-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
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