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Title: Microbial quality and molecular identification of cultivable microorganisms isolated from an urban drinking water distribution system (Limassol, Cyprus)
Authors: Botsaris, George 
Kanetis, Loukas 
Slaný, Michal 
Parpouna, Christiana 
Makris, Konstantinos C. 
Major Field of Science: Natural Sciences
Field Category: Earth and Related Environmental Sciences
Keywords: 16SrRNA;Microbial quality;PCR;Urban water
Issue Date: 11-Nov-2015
Source: Environmental Monitoring and Assessment, 2015, vol. 187, no. 12, pp.10.
Volume: 187
Issue: 12
Start page: 10
End page: 10
DOI: 10.1007/s10661-015-4957-9
Journal: Environmental Monitoring and Assessment 
Abstract: Microorganisms can survive and multiply in aged urban drinking water distribution systems, leading to potential health risks. The objective of this work was to investigate the microbial quality of tap water and molecularly identify its predominant cultivable microorganisms. Tap water samples collected from 24 different households scattered in the urban area of Limassol, Cyprus, were microbiologically tested following standard protocols for coliforms, E. coli, Pseudomonas spp., Enterococcus spp., and total viable count at 22 and 37 °C. Molecular identification was performed on isolated predominant single colonies using 16SrRNA sequencing. Approximately 85 % of the household water samples were contaminated with one or more microorganisms belonging to the genera of Pseudomonas, Corynebacterium, Agrobacterium, Staphylococcus, Bacillus, Delftia, Acinetobacter, Enterococcus, Enterobacter, and Aeromonas. However, all samples tested were free from E. coli. This is the first report in Cyprus molecularly confirming specific genera of relevant microbial communities in tap water.
ISSN: 0167-6369
Rights: © Springer
Attribution-NonCommercial-NoDerivs 3.0 United States
Type: Article
Affiliation : Cyprus University of Technology 
Veterinary Research Institute 
Appears in Collections:Άρθρα/Articles

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